用细胞学方法评价7种抗心律失常西药的寒热药性  

Evaluation of the hot and cold characteristics of seven antiarrhythmic drugs by cytological method

作　　者：王思文[1] 刘建利[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《中华中医药杂志》2015年第4期1053-1057,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.20872118;No.30070905);陕西省重大科技专项项目(No.2008ZDKG-67);陕西省重点实验室基金项目(No.08JZ74;No.2010JS097;No.11JS090;No.12JS110)~~

摘　　要：目的:评价7种抗心律失常西药的寒热药性。方法:用噻唑蓝(MTT)比色法检测7种抗心律失常西药对SMMC7721以及MCF-7细胞体外增殖的影响,并用倒置显微镜观察其对细胞形态特征的影响。结果:盐酸地尔硫卓、盐酸维拉帕米、盐酸胺碘酮、盐酸普罗帕酮的药性为寒性;酒石酸美托洛尔、富马酸比索洛尔、卡维地洛为热性。形态学观察表明盐酸地尔硫卓、盐酸维拉帕米、盐酸胺碘酮、盐酸普罗帕酮使细胞密度减小,固缩变圆;酒石酸美托洛尔、富马酸比索洛尔、卡维地洛在低浓度时可使细胞密度增加,结构致密、生长旺盛。结论:细胞学方法能够用于西药寒热药性的评价,为西药中药化提供了一种简单实用的评价寒热药性的方法。Objective： To evaluate the hot and cold characteristics of 7 antiarrhythmic drugs.Methods： MTT assay was used to investigate the effect of 7 antiarrhythmic drugs on the growth and proliferation of SMMC7721 cells and MFC-7 cells in vitro.Morphological changes were observed with inverted microscope.Results： Diltiazem hydrochloride,verapamil hydrochloride,amiodarone hydrochloride,propafenone hydrochloride showed cold or cool characteristics,and metoprolol tartrate,bisoprolol fumarate,carvedilol showed hot or warm characteristics.Morphological observation demonstrated that cells treated with diltiazem hydrochloride,verapamil hydrochloride,amiodarone hydrochloride,propafenone hydrochloride showed lower cell density and rounder pyknosis.The cells treated with metoprolol tartrate,bisoprolol fumarate,carvedilol showed higher cell density,tighter cell structure and more vigorous growth.Conclusion： The cytological method could be used to evaluate the hot and cold characteristics of western drugs,which may provide a simple and practical evaluation method for Chinese medicalization of western drugs.

关 键 词：西药中药化 抗心律失常西药 MTT法 SMMC7721细胞 MCF-7细胞 寒热药性 

分 类 号：R96[医药卫生—药理学]