肾主骨理论与雌激素介导的骨组织端粒酶逆转录酶增龄性变化之关系研究  

Relationship between kidneys governing bone and estrogen-mediated bone tissue telomerase reverse transcriptase aging changes

作　　者：林煜[1] 张怡元[1] 吴银生[2] 肖莉莉[1] 冯尔宥[1] 林燕萍[2] 

机构地区：[1]厦门大学福州第二医院骨科,福州350007 [2]福建中医药大学中西医结合研究院骨病研究所,福州350108

出　　处：《中华中医药杂志》2015年第4期1066-1070,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81302986;No.81001531)~~

摘　　要：目的:通过实验研究探讨"肾主骨"理论和"雌激素介导的骨组织端粒酶逆转录酶增龄性变化"的关系。方法:收集不同年龄段不同性别符合纳入标准、排除标准的人的松质骨,采用Real-time PCR、Western Blot等方法检测各年龄段人群的骨组织中的端粒酶逆转录酶(TERT)、雌激素受体(ER)的表达和水平,同时采用ELISA检测血清中的骨钙素(BGP)、抗酒石酸酸性磷酸酶5b(TRACP5b)、雌二醇(E2)、睾酮(T)含量。结果:男性与女性组骨组织中TERT、ERα的mRNA表达均随年龄的增长而降低(P<0.05)。各组TERT蛋白表达随着年龄增加逐渐减少(P<0.05)。男性与女性血清中的E2、T、BGP水平均随着年龄增大逐渐降低(P<0.05)。TRACP5b随着年龄增大逐渐升高(P<0.05)。其中女性绝经前后E2、BGP、TRACP5b有显著性变化(P<0.01)。结论:在自然衰老过程中,体内雌激素水平下降的同时,骨组织内TERT转录水平降低,端粒酶活性下降,使骨组织中骨形成功能衰退,骨形成低于骨吸收,从而导致骨质疏松,符合中医"肾主骨"理论。Objective： To explore the relationship between kidneys governing bone and estrogen-mediated bone tissue telomerase reverse transcriptase aging changes.Methods： Collected male and female patients of cancellous bone,which met the inclusion criteria and exclusion criteria.TERT,ER in bone tissue expression were measured by ELISA.Results： ERα,TERT＇s mRNA and protein expression in bone tissue of male and female were increased with age and decreased,the group had significant difference（P〈0.05）.ELISA assay results showed： male,female serum BGP,E2,T average increased as the age gradually decreased（P〈0.05）,TRACP5 b water average increased along with the age increased gradually（P〈0.05）.Women before and after menopause ER,BGP,TRACP5 b significant changed（P〈0.01）.Conclusion： A natural aging process,while estrogen levels decline,bone tissue reduction in the transcriptional level in TERT,the telomerase activity decreases,bone formation is lower than the bone resorption,resulting in osteoporosis.It accordes traditional Chinese medicine theory ‘kidneys governing bone＇.

关 键 词：雌激素 端粒酶逆转录酶 骨衰老 增龄 肾主骨 

分 类 号：R259[医药卫生—中西医结合]