麦门冬汤诱导人肺腺癌A549细胞凋亡作用及其机制  

Research on A549 cell apoptosis mechanism induced by Maimendong Decoction

作　　者：蒋时红[1] 孙超龙 刘燕[2] 张文娴[1] 

机构地区：[1]河南中医学院,郑州450046 [2]湖北中医药大学,武汉430065

出　　处：《中华中医药杂志》2015年第4期1236-1238,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河南省基础与前沿技术研究项目(No.112300410030);河南省教育厅科学技术研究重点项目(No.14A360019);河南中医学院创新团队支持计划(No.2013-113)~~

摘　　要：目的:研究麦门冬汤对人肺腺癌A549细胞凋亡的影响。方法:采用噻唑蓝(MTT)法检测麦门冬汤(0.25、2.5、25g/L)作用24、48、72h后人肺腺癌A549细胞(2×104个/m L)活性;吖啶橙(AO)/EB双荧光染色观察麦门冬汤各剂量作用48h后A549细胞凋亡形态学改变;流式细胞术分析麦门冬汤作用48h后A549细胞凋亡率;Western Blot检测麦门冬汤对凋亡相关蛋白表皮生长因子受体(EGFR)、信号转导子和转录激活子3(STAT3)表达的影响。结果:各剂量麦门冬汤能够抑制A549细胞活性,诱导其发生凋亡形态学改变,且A549细胞凋亡率与麦门冬汤剂量呈正相关(P<0.01);Western Blot检测表明低、中剂量麦门冬汤可使EGFR、STAT3表达下降。结论:麦门冬汤可诱导A549细胞凋亡,其作用机制可能与下调EGFR、STAT3蛋白表达相关。Objective： To study the influence of Maimendong Decoction on A549 cell apoptosis.Methods： MTT method was used to test the A549 cell（2×104cells/m L） viability after intervention of Maimendong Decoction（0.25,2.5,25g/L） on the 24 th h,48 th h,72 nd h.AO/EB staining method was used to observe the morphological changes of A549 cells after intervention of Maimendong Decoction on the 48 th hours.The apoptosis rate of A549 cells after intervention of Maimendong Decoction on the 48 th hours was Detected by flow cytometry（FCM）.The protein expression of epidermal growth factor receptor（EGFR）,regulator of G protein signaling and signal transducer and activator of transcription 3（STAT3） was determined by western blot.Results： Maimendong Decoction（0.25,2.5,25g/L） could inhibited the A549 cell viability,and induce the apoptosis morphological changes in A549 cells.There was positive correlation between A549 cell apoptosis rate and the dosage of Maimendong Decoction（P〈0.01）.The results of western blot showed that Maimendong Decoction could decrease the expression of EGFR and STAT3.Conclusion： Maimendong Decoction could induce the apoptosis of A549 cells,and the mechanism might be associated with down-regulation of EGFR and STAT3 protein.

关 键 词：麦门冬汤 人肺腺癌A549细胞 凋亡 表皮生长因子受体 信号转导子和转录激活子3 

分 类 号：R285.6[医药卫生—中药学]