温阳补肾方含药血清对破骨细胞骨吸收功能的影响  

Effects of serum containing Wenyang Bushen Formula on expression of bone resorption in osteoclasts

作　　者：陈泽荣[1] 邱慈鑫 陈晨[1] 杨树华[1] 陈泽华[1] 林海英[1] 李楠[1] 王和鸣[1] 

机构地区：[1]福建中医药大学骨伤学院,福州350122

出　　处：《中华中医药杂志》2015年第4期1245-1248,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81173283);福建省教育厅课题(No.JA13156)~~

摘　　要：目的:探讨温阳补肾方含药血清对破骨细胞骨吸收功能的影响。方法:体外诱导破骨前体细胞系RAW264.7细胞向破骨细胞分化,分别用温阳补肾方低、中、高剂量组含药血清和0.9%氯化钠溶液血清干预,通过抗酒石酸酸性磷酸酶(TRACP)酶动力法测定各组TRACP活性,实时荧光定量PCR检测温阳补肾方对破骨细胞组织蛋白酶K(Cts K)、基质金属蛋白酶9(MMP-9)mRNA水平。结果:与对照组比较,温阳补肾方含药血清可显著抑制破骨细胞TRACP活性(P<0.01),显著降低破骨细胞骨Cts K、MMP-9 mRNA的表达(P<0.01)。结论:温阳补肾方含药血清可抑制破骨细胞的骨吸收功能,中剂量组效果最佳。Objective： To explore the effects of serum containing Wenyang Bushen Formula on expression of bone resorption in osteoclasts.Methods： The preosteoclasts of RAW264.7 cell were induced to mature osteoclast by RANKL and M-CSF.Then cultured the mature osteoclast and the Wenyang Bushen Formula,low,medium and high dose group,and normal saline（NS） contained serum were respectively added.Vigour of serum TRACP were measured by biochem istry for cell culture fluid.The expression of Cts K and MMP-9 mRNA was measured by real time QPCR.Results： Compared with control group,Wenyang Bushen Formula group can significantly inhibited the TRACP activities（P〈0.01）.The Expression of Cts K and MMP-9 in osteoclast of Wenyang Bushen group was significantly lower than NS group（P〈0.01）.Conclusion： Wenyang Bushen Formula can effectively inhibit the expression of bone resorption in osteoclasts,and the effects of Wenyang Bushen Formula medium dose group is the best.

关 键 词：温阳补肾方 破骨细胞 骨吸收 

分 类 号：R285[医药卫生—中药学]