蝎毒多肽对白血病细胞株KG1a干细胞活性的影响  

Study on the effects of PESV on the viability of leukemia stem cells line KG1a

作　　者：杨向东[1] 李红玉[2] 李德冠[3] 史哲新[1] 杨文华[1] 颜田赅[1] 闫理想 王兴丽[1] 

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津中医药大学,天津300193 [3]中国医学科学院放射医学研究所,天津300192

出　　处：《中华中医药杂志》2015年第4期1278-1281,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：天津市教委项目(No.20100206);天津市科委青年基金项目(No.12JCQNJC09000)~~

摘　　要：目的:探讨蝎毒多肽对白血病细胞株KG1a干细胞活性的影响。方法:免疫磁珠法分离出CD34+CD38-的KG1a细胞,按照不同给药方式分空白组、PESV组、DNR组、PESV+DNR组,培养后经WST-8、流式细胞仪分别检测KG1a干细胞增殖抑制率、细胞凋亡率和细胞周期,并经RT-PCR检测PTEN基因、Tie-2基因mRNA表达。结果:KG1a干细胞在PESV组、DNR组细胞增殖抑制率(%)与空白组相比差异有统计学意义(P<0.05)。诱导细胞凋亡作用强弱:PESV+DNR组>DNR组>PESV组。细胞周期检测结果显示,G0/G1期PESV+DNR组与PESV组、DNR组相比差异有统计学意义(P<0.01)。在G2/M期PESV+DNR组与PESV组、DNR组相比差异有统计学意义(P<0.05)。PESV组、DNR组、PESV+DNR组KG1a干细胞的PTEN基因、tie-2基因表达均上调,PESV+DNR组为高表达。结论:PESV能够抑制KG1a干细胞增殖,但不能增强DNR的抑制作用;PESV对DNR损伤KG1a干细胞有诱导凋亡和分化的作用,其机制可能与上调KG1a干细胞的PTEN基因、tie-2基因表达有关。Objective： To study the effects of PESV on the viability of leukemia stem cells line KG1a.Methods： The leukemia stem cells line CD34＋CD38-KG1a were isolated by using magnetic activated cells sorting skill.According to different interventions,these cells were divided into four groups： blank group,PESV group,DNR group,and PESV＋DNR group.After cultivation,cellular proliferation inhibition rate,apoptosis rate and cells cycle of leukemia stem cells KG1a were respectively detected by WST-8 and flow cytometry,the gene PTEN and tie-2 mRNA expression were tested by RT-PCR.Results： Compared with the blank group,the proliferation inhibition rate of leukemia stem cells line KG1a in PESV group,DNR group had a statistical significance（P〈0.05）,there was no significant difference in DNR group and PESV＋DNR group（P〈0.05）.The results of cells cycle detection showed that there were significant differences in PESV＋DNR group compared with PESV group and DNR group in G0/G1 period（P〈0.01）,there were significant differences in PESV＋DNR group compared with PESV group and DNR group in G2/M period（P〈0.05）.Gene PTEN and tie-2 expression were all upgraded in PESV group,DNR group and PESV＋DNR group,which was the highest expression in PESV＋DNR group.Conclusion： PESV could restrain the proliferation of leukemia stem cells line KG1a,but couldn＇t enhance the inhibition effect of DNR,PESV had a role in inducing apoptosis and differentiation on leukemia stem cells line KG1a after injured by DNR,the mechanism might be connected with the up-regulation of gene PTEN and tie-2 expression in leukemia stem cells line KG1a.

关 键 词：白血病干细胞 蝎毒多肽 KG1a细胞 增殖 凋亡 周期 tie-2基因 

分 类 号：R285[医药卫生—中药学]