辛夷对急性酒精性肝损伤小鼠的肝保护作用及机制研究  

Protective effect of Magnolia biondii Pamp on the acute alcoholic liver injury in mice and study its mechanisms

作　　者：黄川锋[1] 王海鑫[1] 康爱英[1] 张国良[1] 焦志峰[1] 丁可[1] 

机构地区：[1]南阳医学高等专科学校基础医学部,河南南阳473003

出　　处：《中国临床药理学杂志》2015年第7期515-518,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河南省教育厅自然科学计划基金资助项目

摘　　要：目的研究辛夷对急性酒精性肝损伤小鼠肝抗氧化功能和组织病理改变的影响及可能机制。方法将50只雄性昆明种小鼠随机分为5组:空白组、模型组和低、中、高剂量辛夷组(1.0,2.0,4.0 g·kg-1),各组连续灌胃10 d。用白酒(12m L·kg-1)灌胃法建立急性酒精性肝损伤小鼠模型。测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;测定肝组织丙二醛(MDA)含量和谷胱甘肽过氧化物酶(GSH-PX)活性,实时荧光定量PCR检测肝组织细胞色素氧化酶P4502E1(CYP2E1)mRNA表达水平。肝组织HE染色观察病理改变。结果和模型组比较,辛夷中、高剂量组小鼠血清ALT、AST活性和肝组织MDA含量、CYP2E1mRNA表达水平显著降低,而血清GSH-PX含量明显增高(均P<0.05),肝细胞变性、坏死、炎性细胞浸润程度明显减轻。结论辛夷对小鼠酒精性肝损伤具有明显的保护作用,可能与降低CYP2E1 mRNA表达水平和氧化应激作用有关。Objective To study the effect of Magnolia biondii Pamp （ MBP ） on hepatic antioxidant function and pathological histology for alcoholic liver injury in mice.Methods Fifty male KM mice were ran-domly divided into five groups as below：blank group , acute alcohol liver injury model group , MBP low -, medium -and high -dose groups （1.0, 2.0, 4.0 g· kg -1 ）.The acute alcohol liver injury mouse model was set up by intragastric administration of alcohol once daily for 10 days.The activity of serum alanine aminotransferase （ ALT ） , aspartate aminotransferase （ AST ） , glutathione peroxidase （ GSH -PX ） activity and malondialdehyde （ MDA） content in liver were measured.The ex-pression of cytochrome P450 2E1 （CYP2E1） mRNA from the liver were determined by real-time fluorescent quantitative PCR.The pathological changes of liver were observed by light microscope with HE staining.Results Compared with the model group , MBP medium -and high-dose groups have little pathological damage of liver.The levels of ALT, AST, MDA and CYP2E1 mRNA were significant decreased while GSH-PX was significant increased.Conclusion MBP have a signifi-cant protective effect on acute alcohol liver injury in mice , which exerted its beneficial effects mainly through inhibiting oxidative damage and de-creased the expression of CYP 2E1 mRNA.

关 键 词：辛夷 酒精性肝损伤 氧化应激 细胞色素P4502E1 

分 类 号：R285.5[医药卫生—中药学] R965.1[医药卫生—中医学]