七氟醚对新生鼠海马N-甲基-D-天冬氨酸受体亚基mRNA表达的影响  

Effect of sevoflurane exposure on mRNA expression of N-methyl-D-aspartate receptor subunit in neonatal rat hippocampi

作　　者：卢国林[1] 陈小琳[1] 张素晶[1] 毛晓丹[2] 廖鹨[3] 

机构地区：[1]福建省妇幼保健院麻醉科,福州350001 [2]福建省妇幼保健院妇科肿瘤实验室,福州350001 [3]福建省妇幼保健院病理科,福州350001

出　　处：《中国临床药理学杂志》2015年第7期548-550,共3页The Chinese Journal of Clinical Pharmacology

基　　金：福建省卫计委青年科研基金资助项目(2012-1-9)

摘　　要：目的探讨七氟醚对新生大鼠海马N-甲基-D-天冬氨酸受体(NMDA)亚基mRNA表达的影响。方法新生SD大鼠随机分为2组(均12只),七氟醚组吸入3%七氟醚6 h,而对照组吸入30%氧气6 h。2组大鼠吸入气体后24 h处死,取海马组织,用原位杂交法检测海马NMDA受体亚基mRNA表达及NMDA受体2B亚基(NR2B)与NMDA受体2A亚基(NR2A)比率的变化。结果与对照组比较,七氟醚组NR2AmRNA表达显著下调(P<0.05),NR2BmRNA表达显著上调(P<0.05),NR2B/NR2A的比率显著上升(P<0.05)。结论七氟醚诱发新生鼠海马神经毒性可能与调控NMDA受体亚基mRNA表达有关。Objective To investigate the effect of sevoflurane on mRNA expression of N -methyl -D -aspartate （ NMDA ） receptor subunit in neonatal rat hippocampi.Methods Twenty-four SD rats were devided into 2 groups（ n=12 each） via a random number table：sevoflurane group and control group.The 3.0% sevoflurane and oxy-gen inhalated for 6 h respectively.Rats were sacrificed at 24 h after inhalation of sevoflurane or oxygen.Hippocampi were removed to idendtify the mRNA expression of NMDA subunit and the ratio of NR2 B/NR2 A through in situ hybridization.Results Compared with control group, NR2BmRNA and the ratio of NR2B/NR2A increased, and NR2 AmRNA decreased significantly in sevoflurane group （ P〈0.05 ）.Conclusion Neurotoxicity of sevoflurane in immature hippocampi might be attributed to regulation of mRNA expression of NMDA subunit in neonatal rat.

关 键 词：麻醉药 吸入 N-甲基-D-天冬氨酸受体 原位杂交 海马 

分 类 号：R971.2[医药卫生—药品] R965.1[医药卫生—药学]