抵抗素299G/A和过氧化物酶体激增物激活受体γPro12A1a基因多态性与妊娠期糖尿病的相关性研究  

Study on the correlation of resistin299G/A and PPARγProl2Ala gene polymorphism with gestational diabetes mellitus

作　　者：晏益民[1] 杨晓红[1] 管晓峰[1] 黄淑玉[1] 邹毅[1] 吴敏[1] 徐焱成[2] 孙家忠[2] 

机构地区：[1]孝感市中心医院内分泌科,432000 [2]武汉大学中南医院内分泌科

出　　处：《中国糖尿病杂志》2015年第4期306-309,共4页Chinese Journal of Diabetes

摘　　要：目的探讨抵抗素299G/A、PPARγPro12Ala基因多态性与GDM的相关性。方法选取156例GDM孕妇为GDM组和180名正常对照(NC)孕妇为NC组,采用聚合酶链反应-限制性内切酶技术(PCR-RFLP)对基因多态性进行检测。结果 GDM、NC组抵抗素299G/A基因型频率GG∶GA∶AA分别为41.3%∶46.3%∶11.6%、35.2%∶51.7%∶13.8%,PPARγPro12Ala基因型频率PP∶PA∶AA分别为92.3%∶7.7%∶0.0%、85.0%∶13.3%∶1.7%,两组间基因型和等位基因频率比较差异均无统计学意义。GDM组抵抗素基因GA、AA基因型孕妇FIns、胰岛素抵抗指数(HOMA-IR)高于GG基因型[(12.99±9.98)vs(8.06±2.25)mU/L,(18.35±6.32)vs(8.06±2.25)mU/L;(2.45±0.96)vs(1.31±0.36),(3.45±1.89)vs(1.31±0.36),P<0.05]。PPARγ基因PP基因型孕妇BMI高于PA基因型[(29.47±1.68)vs(23.89±2.88)kg/m2,P<0.01]。结论抵抗素299G/A、PPARγPro12Ala基因多态性与GDM发病无相关性,可能与GDM患者肥胖、IR有关。Objective To investigate the correlation of resistin299G/A ,PPARγPro12Ala polymorphism with gestational diabetes mellitus （GDM ）. Methods Selecting 156 cases of pregnant women with GDM as GDM group ,and 180 cases of normal control （NC） pregnant women as NC group , polymerase chain reaction and restriction endonuclease technique （PCR‐RFLP ） were employed for genotyping resistin299G/A ,PPARγPro12Ala polymorphisms. Results Resistin299G/A polymorphism genotype frequencies of GG∶GA∶ AA were 41.3% ∶46.3% ∶11.6% respectively for GDM group and 35.2% ∶51.7% ∶13.3% for NC group.PPARγPro12Ala polymorphism genotype frequencies of PP：PA ：AA were respectively 92.4% ∶7.6% ∶0.0% for GDM group and 85.0% ∶13.3% ∶1.7% for NC group.There was no difference between two groups of genotype and allele frequency. The levels of FIns and HOMA‐IR in GDM groupwere higher in resistin GA and AA genotypes than in GG genotype [FIns（12.99 ± 9.98） vs （8.06 ± 2.25）mU/L ,（18.35 ± 6.32） vs （8.06 ± 2.25）mU/L ,respectively ;HOMA‐IR（2.45 ± 2.32） vs （1.31 ± 0.36） ,（3.45 ± 1.89） vs （1.31 ± 0.36） ,respectively ;all P〈0.05]. BMI in GDM group was significantly higher in PP genotype than in PA genotype [（29.47 ± 1.68） vs （23.89 ± 2.88）kg/m2 , P〈0.01]. Conclusion Resistin299G/A and PPARγPro12Ala polymorphism may have no relationship with the incidence of GDM ,but may have a certain relationship with obesity and IR.

关 键 词：糖尿病 妊娠 抵抗素 过氧化物酶体激增物激活受体γ 

分 类 号：R714.256[医药卫生—妇产科学]