成年鼠糖尿病自发康复时胰岛β细胞雄激素受体表达的研究  

Androgen receptor expression on islet β cells during spontaneous recovery in diabetes adult mice

作　　者：梁同衍 王丽红[2] 周生伟[3] 杜红梅[3] 李如江[3] 

机构地区：[1]潍坊市市立医院内二科,261021 [2]潍坊市人民医院产前诊断科 [3]潍坊医学院组织学与胚胎学教研室

出　　处：《中国糖尿病杂志》2015年第4期360-364,共5页Chinese Journal of Diabetes

基　　金：山东省自然科学基金(2009ZRB019FB);山东省高等学校科技计划项目(J12LK05);潍坊医学院科技创新基金(K11TS1010)

摘　　要：目的观察成年鼠糖尿病康自发复时胰岛β细胞特异基因的表达。方法建立自发康复糖尿病成年小鼠模型。在糖尿病自发康复过程中,以流式细胞术分选其胰岛β细胞,通过mRNA差异显示法研究胰岛β细胞基因差异表达,以免疫荧光双标显示其蛋白差异表达。结果在糖尿病自发康复过程中,以mRNA差异显示法在胰岛β细胞内共检出12个差异表达基因,其中,雄激素受体(AR)在糖尿病最严重时低表达甚至不表达,在糖尿病康复过程中表达增强,在糖尿病完全康复时高表达(P<0.01)。结论以mRNA差异显示法研究胰岛β细胞特异基因的表达切实可行。糖尿病康复时,胰岛β细胞雄激素受体表达与功能性胰岛β细胞的增多密切相关。Objective To observe the specific gene expressions of islet β cells during spontaneous recovery in diabetic adult mice. Methods A diabetic adult mouse with spontaneous recovery was established. Pancreaticβcells of adult mice with recovery from diabetes were sorted by flow cytometry , then differential mRNA expressions ofβcells were analyzed among groups via mRNA differential display , and their protein expressions were determined by double immunofluorescence. Results In the process of diabetes spontaneous recovery ,12 differential expression genes were obtained from pancreatic β cells by mRNA differential display ,and AR expression inβcells was decreased significantly ,even disappeared when the diabetes was the most serious. However ,it gradually increased with recovery from diabetes ,and was in a higher level when diabetes was recovered completely （P〈 0.01）. Conclusion mRNA differential display is a powerful tool to study the differential gene expression in pancreaticβcells. The change of AR expression inβcells is strongly associated with an increase in the number of functional pancreatic βcells with recovery from diabetes.

关 键 词：糖尿病 胰岛Β细胞 MRNA差异显示 雄激素受体 

分 类 号：R587.1[医药卫生—内分泌]