SERS标记方法在生化分析中的应用  

SERS Label Method and its Application in the Biochemical Analysis

作　　者：刘建芝[1] 朱桂池[1] 胡勇军[1] 

机构地区：[1]华南师范大学生物光子学研究院激光生命科学教育部重点实验室,广东广州510631

出　　处：《激光生物学报》2015年第1期17-24,共8页Acta Laser Biology Sinica

基　　金：国家自然科学基金面上项目(21273083;U1332132);广东省自然科学科学基金项目(S2013010016551)

摘　　要：表面增强拉曼散射(SERS)标记方法结合现代生物标记方法与SERS光谱技术,使吸附到金或银等贵金属表面的标记分子的拉曼信号显著增强,并将其作为标记示踪信号,具有生物兼容性好、灵敏度高、分子特征性强和快速简便等优点,已成为新颖的标记示踪技术的研究热点之一。本文综述近年来SERS标记技术应用于基因分析、蛋白质检测、微生物检测、肿瘤靶向和小分子物质的最新进展,着重介绍蛋白质和小分子物质的检测,并展望了今后的发展方向。Surface-enhanced Raman scattering （SERS）label method is a novel bioassay technology,which combines the modern bio-label technology and SERS spectroscopy.In present method,the signal of the Raman report mole cules, acting as the label tracer signal,is enhanced by adsorbing on the noble metal （i.e.Ag and Au）nanoparticles.With the advantages of high sensitivity,high specificity,fast and easy detection in biochemical analysis,the study on SERS label method has been a hot topic.Here,SERS labeling method has been introduced firstly and its applications recently in the labeled immunoassay,tumor targeting,DNA molecular recognition and microbiological detection have been reviewed.At last,future prospect in this field are indicated.

关 键 词：表面增强拉曼散射 标记 基因 蛋白质 微生物 肿瘤靶向 小分子 

分 类 号：O657.37[理学—分析化学]