丹参酮Ⅰ抑制小鼠B16黑色素瘤细胞研究  

Experiment on Inhibitory Effect of TanshinoneⅠAgainst Mouse B16 Melanoma Cells

作　　者：张蕾[1,2] 陈璐[3] 李蒙蒙[3] 金王东 王飘逸 单乐天[3] 

机构地区：[1]浙江科技学院生物与化学工程学院,浙江杭州310023 [2]浙江省农产品化学与生物加工技术重点实验室,浙江杭州310023 [3]浙江中医药大学,浙江杭州310053

出　　处：《浙江林业科技》2014年第3期14-18,共5页Journal of Zhejiang Forestry Science and Technology

基　　金：国家自然科学基金青年项目(81302989);高等学校博士学科点专项科研基金(20133322120006)

摘　　要：将丹参酮Ⅰ(含量为90%)配制成不同浓度作用于体外培养的B16细胞,对B16细胞用MTT法检测药物对细胞生长抑制率,收集并采用PI染色,观察细胞的形态,通过流式细胞术(FCM)检测细胞周期及凋亡率。结果表明:不同质量浓度的(5、4、3μg/mL)丹参酮I对B16细胞的抑制作用均有明显的剂量和时间效应;丹参酮I可以使B16细胞周期的构成发生明显的变化;丹参酮I对B16细胞的生长有明显的抑制作用,作用机制之一是使S期细胞的比例降低,促使细胞凋亡。Experiments were conducted on effect of different concentrations of Tanshinone I (90%) against in vitro cultured mouse B16 melanoma cells. Cell growth inhibition rate was determined by MTT method. Treated cells were observed, collected and stained by PI. Cell cycles and apoptosis rate were detected by flow cytometry (FCM). Results demonstrated that different concentration (5, 4, 3μg /ml) of Tanshinone I had evident inhibitory effect on B16 melanoma cells with dosage and duration, and the composition of B16 cell cycles changed.

关 键 词：丹参酮I 抑制增殖 B16黑色素瘤 抗肿瘤 

分 类 号：R96[医药卫生—药理学]