食物限制对黑线仓鼠免疫功能的影响  

Effect of food restriction on immune function in the striped hamster( Cricetulus barabensis)

作　　者：徐德立[1] 徐来祥[1] 

机构地区：[1]曲阜师范大学生命科学学院,曲阜273165

出　　处：《生态学报》2015年第6期1882-1890,共9页Acta Ecologica Sinica

基　　金：国家自然科学基金(31070332;31370427);山东省自然科学基金(ZR2013CM019);曲阜师范大学博士启动基金(bsqd20100204)

摘　　要：温带地区小型哺乳动物经常面临着食物资源的波动。食物对动物的免疫功能具有重要影响。将19只成年雄性黑线仓鼠(Cricetulus barabensis)随机分为自由取食组(n=9)和限食组(n=10)。注射植物血球凝集素(PHA)来测定细胞介导的免疫反应,用匙孔血蓝蛋白(KLH)免疫动物,然后测定抗KLH抗体的浓度以反映其体液免疫功能。旨在检验食物限制是否会抑制黑线仓鼠的细胞免疫和体液免疫功能。结果发现,与对照组相比,限食组黑线仓鼠具有较低的体重、体脂、脾脏鲜重、血清瘦素水平、免疫球蛋白Ig G和Ig M浓度。而限食对胸腺鲜重、白细胞数、皮质酮水平以及PHA反应没有显著影响。结果表明黑线仓鼠免疫系统的不同成分对限食反应存在差异,在食物资源短缺时,黑线仓鼠防御细胞外病原体的能力降低,从而导致生存能力的下降。The immune system protects animals against environmental pathogens,and hence it plays an important role in their survival and fitness. However,immune function is influenced by many factors including food availability. Small mammals in the temperate zone often face fluctuations in food availability,which is important to their survival. Changes in food availability may have a great influence on an animals＇ immunity. Some investigators have examined the effect of reduced food availability（ i. e.,food restriction） on the immune function in small rodents,but their results are often inconsistent. Food restriction could depress,enhance or have no effect on immunity in different species. In order to clarify the discrepancy between results,the striped hamster（ Cricetulus barabensis） was used to examine the effect of food restriction on its immune function. The striped hamster is a major pest rodent in northern China. It is granivorous,nocturnal and feeds mainly on plant stems and leaves during summer and forages crop seeds in winter. The climate is arid and characterized by warm and dry summers and cold winters. Thus,the species experiences great seasonal fluctuations in temperature,photoperiod as well as food quality and availability. The question we focused on in the present study was how the immune function would vary in the face of reduced food availability for hamsters. Nineteen adult male hamsters were randomly divided into the fed ad libitum group（ Fed,n = 9） and food restricted group（ FR,n = 10）（ 90% of baseline food intake）. The course of food restriction was 21 days. Hamsters were injected with phytohaemagglutinin（ PHA） and keyhole limpet haemocyanin（ KLH） solution to assess cellular and humoral immunity,respectively. Our goal was to testwhether food restriction would suppress cellular and humoral immunity in striped hamsters. Body mass in the FR group decreased significantly after 21 days of food restriction,whereas body mass in the Fed group did not change significantly.Body mass

关 键 词：食物限制 体液免疫 黑线仓鼠(Cricetulus barabensis) 植物血球凝集素反应 

分 类 号：S852.4[农业科学—基础兽医学]