雄激素受体基因腺病毒载体的构建及对人毛乳头细胞增生的影响  

Construction of AR adenoviral vectors and its effects on proliferation of human dermal papilla cells

作　　者：兰雪梅[1] 谭欢[1] 钟白玉[1] 冯林[1] 杨希川[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038

出　　处：《实用皮肤病学杂志》2015年第2期81-84,共4页Journal of Practical Dermatology

基　　金：国家自然科学基金资助项目(81271771)

摘　　要：目的检测未转染的毛乳头细胞第3、6、9代雄激素受体(androgen receptor,AR)蛋白的表达;构建及鉴定AR基因过表达的腺病毒载体,将其转染进入人毛乳头细胞(dermal papilla cell,DPC),探讨AR基因对人DPC增生的影响。方法应用基因同源重组获得p Adeasy-Adtrack-AR重组腺病毒质粒,腺病毒感染人DPC,采用蛋白质印迹法(Western blot)检测AR的表达,应用CCK-8法检测人DPC的增生。结果未转染前,DPC的AR表达量随着传代逐渐降低;成功构建了AR基因腺病毒载体,腺病毒转染人DPC 48 h后AR蛋白高表达,腺病毒转染24、48、72 h对人DPC的增生无影响。结论人DPC过表达AR基因模型为进一步研究雄激素性脱发提供了一定的实验基础。Objective To detect the expression of androgen receptor（AR） before transfection; and to construct the adenoviral overexpressing vectors of AR and investigate its effects on proliferation of human dermal papilla cells（DPCs） after transfection. Methods The AR adenoviral vectors were constructed by using gene recombination technique, then the expression of AR was detected by western bolt, and the vectors＇ effects on proliferation of DPCs was detected by CCK-8 assay. Results Before transfection, AR expression was gradually reduced with the passage of DPCs. The AR adenoviral vectors were successfully constructed. The AR expression in DPCs was increased after transfection. The AR adenoviral vectors did not effect the proliferation of DPCs. Conclusion It＇s important to construct an AR overexpressing model, which provides some experimental basis for further study of alopecia androgenetic.

关 键 词：受体 雄激素 脱发 雄激素性 毛乳头细胞 

分 类 号：R758.71[医药卫生—皮肤病学与性病学]