补骨脂素和女贞子对小鼠B16黑素瘤细胞c-KIT表达的影响  

The effects of malaytea scurfpea fruit and fructus ligustri lucidi on the migration and c-KIT expression of B16 melanoma cells

作　　者：霍晶[1] 宋新峰[2] 

机构地区：[1]德州市人民医院皮肤科,德州253014 [2]德州学院医药与护理学院

出　　处：《实用皮肤病学杂志》2015年第2期89-91,94,共4页Journal of Practical Dermatology

基　　金：德州市科技发展计划项目(2011C08)

摘　　要：目的探讨中药补骨脂素和女贞子对小鼠B16黑素瘤细胞c-KIT表达的影响及意义。方法体外培养小鼠B16黑素瘤细胞,用反转录-聚合酶链反应(RT-PCR)和蛋白质印迹法(Western blot)测定补骨脂素和女贞子对小鼠B16黑素瘤细胞c-KIT表达的影响,用Transwell小室法检测补骨脂素和女贞子对小鼠B16黑素瘤细胞迁移功能的影响。结果补骨脂素与女贞子用药组迁移到膜下层的每视野细胞个数分别较对照组多(P<0.05),并呈剂量依赖关系;用药组c-KIT m RNA、c-KIT蛋白表达分别比对照组增加,差异均有统计学意义(P<0.05)。结论补骨脂素和女贞子可能是通过促进小鼠B16黑素瘤细胞c-KIT的表达而促进其迁移功能,从而对白癜风产生治疗作用。Objective To study the effects of malaytea scurfpea fruit and fructus ligustri lucidi on the migration and c-KIT expression of mouse melanoma cell line（B16）. Methods B16 cells were treated with malaytea scurfpea fruit and fructus ligustri lucidi respectively, then the cell migration was detected by transwell assay, and c-KIT expression was detected by RT-PCR and western blot. Results Migrated cells in every vision of the three malaytea scurfpea fruit treated groups and the three fructus ligustri lucidi treated groups were more than the corresponding control group respectively, there was a signifi cant difference（P0.05）, and the number of the migrated cells was affected by the concentration of the medicine in a dose dependent manner. Compared with the control group, c-KIT expression of melanoma cells of malaytea scurfpea fruit group（1.0×10-5mmol/L） and fructus ligustri lucidi group（1.0 g/L） was increased respectively, the difference was statistically signifi cant（P0.05）. Conclusion Malaytea scurfpea fruit and fructus ligustri lucidi promoted mouse B16 melanoma cell migration by promoting its c-KIT expression, which may be the mechanism of their therapeutic effects on vitiligo.

关 键 词：补骨脂素 女贞子 B16黑素瘤细胞 c-KIT 糖蛋白 

分 类 号：R285[医药卫生—中药学]