不同树脂对麻黄附子细辛汤中5种生物碱的洗脱性能比较  

Comparison of the elution properties of different resins for five alkaloids in Mahuang-fuzi-xixin decoction

作　　者：曹洪杰[1] 杨彬彬[1] 容蓉[1] 陈迩东 杨勇[1] 巩丽丽[1] 蒋海强[1] 

机构地区：[1]山东中医药大学药学院,济南250355

出　　处：《中国新药杂志》2015年第7期831-836,共6页Chinese Journal of New Drugs

基　　金：国家自然基金项目(81273626);国家"重大新药创制"科技重大专项(2014ZX09509-001001005)

摘　　要：目的:比较不同类型大孔树脂对于麻黄细辛附子汤中麻黄碱、伪麻黄碱、苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱的洗脱性能。方法:采用HPLC-DAD对D101,HPD-100,HPD-722,HPD-400这4种树脂不同洗脱部位中5种生物碱的含量进行测定,并计算其洗脱率。结果:HPD-722型大孔树脂对复方中5种生物碱的富集作用最好,2种麻黄碱和3种单酯型乌头碱分别在30%和50%Et OH洗脱部位具有较高的洗脱率。结论:HPD-722型大孔树脂可用于麻黄附子细辛汤中生物碱类成分的富集纯化。Objective：To compare the elution property of different types of macroporous resins for ephed- rine, pseudoephedrine, benzoylaconine, henzoylmesaconitine and benzoylhypaconine from Mahuang-fuzi-xixin decoc- tion. Methods ：The contents of the five alkaloids in different elution parts of maeroporous resin of D101, HPD-100, HPD-722 and HPD-400 were determined by high performance liquid chromatography with diode array detector, and the elution rate was calculated. Results：The resin of HPD-722 showed the best enrichment performance for the five alkaloids,among which ephedrine and pseudoephedrine had the highest contents in the 30% ethanol elution part and benzoylaconine,benzoylmesaconitine and benzoylhypaeonine in the 50% ethanol elution part. Conclusion：The macroporous resin of HPD-722 can be used for the purification and enrichment of alkaloids in Mahuang-fuzi-xixin decoction.

关 键 词：麻黄附子细辛汤 大孔树脂 生物碱 高效液相色谱 

分 类 号：R28[医药卫生—中药学]