杂交榛子体细胞胚发生研究  

Study on Somatic Embryogenesis of Corylus heterophylla×C. avellana

作　　者：张玲 

机构地区：[1]黑龙江省林业科学研究所,哈尔滨150081

出　　处：《林业科技》2015年第2期1-4,共4页Forestry Science & Technology

基　　金：黑龙江省森工总局项目(SGJZQ2012002)资助

摘　　要：不同培养基、激素种类及浓度和外植体对杂交榛子体细胞胚直接和间接诱导的影响的结果表明,MS培养基为杂交榛子体细胞胚发生最佳培养基;激素对杂交榛子体细胞胚直接诱导无显著影响,均可诱导成熟植株;筛选出杂交榛子愈伤组织培养最佳培养为:MS+6-BA2.0 mg/L+NAA1.5 mg/L;再生植株分化最佳培养基为:MS+6-BA2.0mg/L+N AA1.5 mg/L转入MS+6-BA 5.0 mg/L+N AA 0.01+GA3 0.5 mg/L;杂交榛子体细胞胚发生以子叶诱导率最高,以龙榛胚性愈伤组织诱导率最高。Different culture medium,hormone type and concentration,and varieties of explant on hybrid hazelnut inductoon effects of direct and indirect somatic embryogenesis weve studied.The results indicated that the medium of MS was optimal medium for embryonic culture. The hormone hadn’t significant different for direct induction on the hybrid hazelnut somatic embryos, can induce mature plants; Screened callus culture of hybrid hazelnut best culture for was MS ＋ 6-BA2.0mg / L ＋ NAA1.5mg / L; Optimal medium of embryonic callus was MS ＋ 6-BA2.0mg / L ＋ NAA1.5mg / L transferred to MS ＋ 6-BA 5.0mg / L ＋ NAA0.01 ＋ GA3 0.5mg / L; Cotyledons as for explants induced the highest rate. The Long hazelnut was the highest species for embryonic callus induction.

关 键 词：平欧杂交榛子 体细胞胚发生 研究 

分 类 号：S664.4[农业科学—果树学] S603.8[农业科学—园艺学]