蛇床子素对海人酸致痫大鼠神经元Caspase-3和Caspase-9蛋白表达的影响  

Effect of Osthole on Expressions of Caspase-3 and Caspase-9 Proteins in Hippocampal Neuron in Kainite- induced Rats

作　　者：谢洪婷 沈阳[2] 曾常茜[1] 

机构地区：[1]辽宁省生物有机化学重点实验室,大连大学医学院,辽宁大连116622 [2]大连市儿童医院,辽宁大连116012

出　　处：《中医药信息》2015年第2期16-18,共3页Information on Traditional Chinese Medicine

基　　金：大连市科学技术基金计划项目(No.2008J21JH005)

摘　　要：目的:观察蛇床子素对海人酸致痫大鼠海马神经元的影响以及对海马神经元Caspase-3和Caspase-9蛋白表达的影响,揭示蛇床子素对海人酸致痫大鼠海马神经元作用的机制。方法:30只SD大鼠随机分成3组,其中对照组10只、海人酸组10只、蛇床子素组10只。结晶紫染色观察海马神经元的形态,免疫组化染色法检测海马神经元Caspase-3和Caspase-9蛋白的表达。结果:结晶紫染色结果显示,海人酸组中海马神经元胞体皱缩,形态不规则。蛇床子素干预组中海马神经元的数量和形态与对照组相似,胞浆清晰淡染,核仁清楚。免疫组化染色结果显示,海人酸组中海马神经元活性Caspase-3和Caspase-9蛋白表达与对照组比较显著增加(P<0.05),蛇床子素组中海马神经元活性Caspase-3和Caspase-9蛋白表达与海人酸组比较显著减少(P<0.05)。结论:蛇床子素对海人酸致痫大鼠海马神经元具有保护作用,其机制可能与蛇床子素抑制海马神经元Caspase-3和Caspase-9蛋白活性有关。Objective： To investigate the effect of osthole on the hippocampal neuron and the expressions of Caspase - 3 and Caspase - 9 protein in the hippocampal neuron in kainite - induced rats. Methods ： 30 SD rats were divided into 3 groups,with 10 rats in each group, including a control group, a kainite group and an ost- hole group. Crystal violet staining was used to observe the morphological change of the hippocampal neuron. Immunohistochemistry staining was used to examine the expressions of Caspase -3 and Caspase -9 protein in the hippocampal neuron. Results： Crystal violet staining showed that the neurons of the hippocampal region exhibited irregular morphology and body shrinked, and reduced in numbers in the kainite group. However, the neurons in the osthole group showed clear cytoplasm dyed and nucleoli and similar number and morphology to the control group. Immunohistochemistry staining showed that the expressions of Caspase - 3 and Caspase - 9 in the hippocampal neuron in the kainite group increased significantly than those in the control group （P 〈 0. 05 ）. The expressions of active Caspase - 3 and active Caspase - 9 of the hippocampal neuron in the Ostholegroup decreased obviously than that in the kainite group （ P 〈 0. 05 ）. Conclusion ： Osthole has a protective effect on the hippocampal neurons in kainite - induced rats by inhibiting the activity of Caspase - 3 and Caspase - 9 protein.

关 键 词：蛇床子素 海人酸 神经元 CASPASE-3 CASPASE-9 

分 类 号：R285.5[医药卫生—中药学]