短瓣金莲花提取物抗炎及抗内毒素作用的实验研究  

Experimental Study on Effects of Anti-inflammatory and Anti-endotoxin Effects of Duanban Jinlianhua (Trollius Ledebouri Deichb) Extracts

作　　者：苏连杰[1] 夏清[1] 张海欧[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040

出　　处：《中国中医药科技》2015年第2期151-152,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：黑龙江省自然科学基金项目(H201321)

摘　　要：目的：观察短瓣金莲花提取物（总黄酮）抗炎及内抗毒素作用;方法：采用小鼠腹腔毛细血管通透性实验;大鼠足跖腱膜下注射100%新鲜鸡蛋清（0.1m L/只）和注射1%甲醛足肿胀实验观察提取物3个剂量组的抗炎作用。采用内毒素1.5mg/kg引起小鼠休克实验,观察短瓣金莲花提取物3个剂量组的抗内毒素作用。结果：短瓣金莲花提取物高、中、低剂量均能显著抑制醋酸所致小鼠毛细血管通透性增高（P〈0.01）;高剂量可显著抑制药后1、2小时的蛋清性足肿胀（P〈0.05、0.01）,中剂量可显著抑制药后2、3小时的蛋清性足肿胀（P〈0.01）,低剂量可抑制药后2小时的蛋清性足肿胀（P〈0.05）;3个剂量对大鼠甲醛性足肿胀在致炎后1~4天均有明显的抑制作用（P〈0.01）,高剂量使内毒素引起小鼠死亡率明显减少（P〈0.01）,中、低剂量对内毒素引起小鼠死亡的保护作用不明显（P〉0.05）。结论：短瓣金莲花提取物具有很好的抗炎及抗内毒素作用。Objective： To observe the anti - inflammatory and anti - endotoxin effects of Trollius ledebouri extract Methods. The experiment of peritoneal capillary permeability in mice ; rats＇ paw swelling induced by egg white/formal dehyde saline solution were used to observe the anti - inflammatory effects of three dose groups from Trollius Chinensis extracts. Mice were injected intraperitoneally endotoxin to observe the anti - endotoxin effect. Results ：Trollius Chinensis extracts（ three dose groups）could significantly inhibit the increasing of capillary per- meabilit by acetic acid （ P 〈 0.01 ） ; high doses could significantly inhibit paw swelling induced by egg white af- ter 1,2 h medication （P 〈 0. 05 to 0. 01 ）, middle dose was after 2,3 h medication （ P 〈 0. 01 ）, and low dose can inhibit paw swelling after 2h medication （P 〈 0.05 ）. Three dose groups significantly inhibited paw swelling induced by formaldehyde after 1 to 4 days medication （P 〈 0.01 ）. High dose significantly reduced mortality in- duced by endotoxin （P 〈 0.01 ） ; middle and low- dose was not obvious （P 〉 0. 05 ）. Conclusion： Trollius ledebouri extract has a good anti -inflammatory and anti -endotoxin effects.

关 键 词：短瓣金莲花提取物(总黄酮) 抗炎作用 抗内毒素作用 

分 类 号：R285.5[医药卫生—中药学]