华蟾素注射液对小鼠肝癌组织Smac及Caspase-9表达的影响  

Effects of Cinobufacini Injection (华蟾素注射液) on Smac,Caspase-9 Expression in Liver Tumor Tissues of Mice's Livers

作　　者：邓德厚[1] 史华[1] 张永军[1] 包文龙[1] 任莉莉[1] 

机构地区：[1]浙江省肿瘤医院中西医结合科,杭州310022

出　　处：《中国中医药科技》2015年第2期153-155,158,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省医药卫生科技计划资助项目(2011KYA036)

摘　　要：目的:观察华蟾素注射液对H22肝癌小鼠肿瘤组织Smac(半胱氨酸)和Caspase-9(天冬氨酸蛋白酶)基因(m RNA)及蛋白表达的影响。方法:40只ICR小鼠采用皮下接种H22肝癌细胞株的方法复制小鼠肝癌模型;造模成功后,将小鼠随机分为4组:模型组,华蟾素注射液低、高剂量组,顺铂(DDP)组;观察各组小鼠肿瘤生长情况变化,采用荧光定量RT-PCR方法和Western Blot方法检测各组H22小鼠肿瘤组织中Smac、Caspase-9基因及蛋白表达。结果:华蟾素低剂量组不能有效抑制肿瘤的生长,而华蟾素高剂量组、DDP组的抑瘤率分别为43.13%、47.06%,具有明显的抑制肿瘤生长作用(P<0.01)。与模型组比较,华蟾素低剂量组小鼠肝癌组织中的Smac、Caspase-9表达无明显差异,而华蟾素高剂量组、DDP组能明显促进肝癌组织中的Smac、Caspase-9的m RNA及蛋白表达(P<0.01)。结论:华蟾素注射液具有抑制肝癌细胞生长的作用,上调肿瘤组织Smac、Caspase-9基因及蛋白的表达,促进肝癌细胞发生凋亡是其部分作用机制。Objective： To investigate the effects of Cinobufacini Injection on Smac、Caspase- 9 genene（ m RNA） and proteinin expressions in tumor tissues of mice bearing Hepatoma H22. Methods： Forty ICR mice were randomly divided into 4 groups： the model group,low,high dosage groups of Cinobufacini Injection,Cisplatin group,after the mice model bearing Hepatoma H22 were established by subcutaneous inoculating tumor cells,tumor growth in mice was observed,the m RNA and protein expressions of Smac,Caspase- 9 in tumour tissues were detected by quantitativereal- time reverse- transcription polymerase chain reaction（ RT- PCR） and Western Blot. Results： The low dosage of Cinobufacini Injection could not inhibit the growth of tumor,the cancer inhibition ratio of high dosage Cinobufacini Injection and Cisplatin group was 43. 13% 、47. 06% respectively,itshowed obvious inhibitory effect on tumor growth（ P〈0. 01）. Compared with the model group,the expression levels of Smac,Caspase- 9 m RNA and protein in low dosage Cinobufacini Injection group has not obvious difference,while the high dosage Cinobufacini Injection and Cisplatingroup could promote the expressions of Smac,Caspase- 9 m RNA and protein（ P〈0. 01） in tumor tissues of mice. Conclusion： Compbifacoinit Injection can inhibi the liver cancer growth by up- regulating the expressions Smac and Caspase- 9,to promote tumor cell apoptosis,and achieve anti- tumor effect.

关 键 词：肝肿瘤 华蟾素注射液 半胱氨酸 天冬氨酸蛋白酶 基因表达 小鼠 

分 类 号：R285.5[医药卫生—中药学]