基于小麦品种‘洛夫林10’的VIGS体系研究  

Study on the VIGS system in wheat(Triticum aestivum L.) cultivar Lovrin10

作　　者：牛笑南 石诚[1] 张路路[1] 刘娜[1] 侯春燕[1] 刘刚[1] 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《河北农业大学学报》2015年第2期7-12,共6页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31171472);高等学校博士学科点专项科研基金资助课题(优先发展领域)(20111302130001);河北省应用基础研究计划重点基础研究项目(12967149D);河北省自然科学基金(C2012204045)

摘　　要：为将病毒介导的基因沉默(Virus induced gene silencing,VIGS)技术应用于小麦-叶锈菌互作体系及小麦基因功能研究,本试验在优化适宜BSMV繁殖并引起发病的环境条件基础上,将病毒载体转染小麦品种‘洛夫林10’(简称L10)叶片并接种叶锈菌生理小种,发现接种病毒对叶锈菌侵染反应型没有影响,通过构建TaCAMTA4与TaATG8的BSMV-VIGS载体,将体外转录病毒转染小麦,以感染BSMV:PDS的L10作指示,在指示叶片变白后,经半定量RT-PCR检测,发现目的基因的表达量明显降低,表明用VIGS技术能够成功沉默L10中的目的基因,这一结果为进一步研究小麦与叶锈菌互作过程中小麦基因的功能及分子机制奠定了基础。To apply the BSMV-induced VIGS in wheat cultivar L10 and investigate the function of genes related to the resistance of leaf rust, the conditions for virus reproduce and infection were optimized. Firstly, the virus of BSMV： 00 was inoculated on L10 and the development of leaf rust was not influenced by BSMV. After that, both of the BSMV： TaCAMTA4 and BSMV： TaATG8 vectors were established and their virus were inoculated on L10, the pheno- type of wheat inoculated BSMV. PDS was observed to indicate the efficiency of VIGS. The semi-quantitative RT-PCR result showed that the expression level of TaCAMTA4 and Ta- ATG8 was decreased significantly. This result revealed that the two genes were silenced suc- cessfully by BSMV-induced gene silencing. This work will lay the foundation for evaluating the function and molecular mechanism of gene related to the resistance of leaf rust.

关 键 词：小麦 大麦条纹花叶病毒(BSMW) 病毒诱导的基因沉默(VIGS) TaCAMTA4 TaATG8 

分 类 号：S512.1[农业科学—作物学]