南宁某警犬基地蜱虫及病原体感染调查  

Investigation of ticks and pathogen infection at a police dog breeding and training base in Nanning

作　　者：王华素 徐金山[2] 彭恒[2] 李孟英[3] 陈要朋[4] 刘铁牛[4] 朱淮民[2] 薛绍礼[1] 

机构地区：[1]安徽医科大学生物医学工程教研室,合肥230032 [2]第二军医大学病原生物教研室,上海200433 [3]解放军第303医院感染科,南宁530021 [4]解放军第303医院检验科,南宁530021

出　　处：《国际医学寄生虫病杂志》2015年第2期94-98,共5页International JOurnal of Medical Parasitic Diseases

基　　金：国家科技重大专项(2012ZX10004-220,2008ZX10004-011);国家自然科学基金(81141085);卫生行业科研专项(201202019)~~

摘　　要：目的 了解广西南宁某警犬养殖训练基地蟀虫及病原体携带情况. 方法 2013年7月在广西南宁某警犬养殖训练基地,采用逆毛式检蜱法采集犬体表以及犬舍墙壁的蜱虫,用巴贝虫属18S rRNA通用引物的巢式PCR、原核生物16S rRNA及真核生物线粒体16S rRNA通用引物的PCR和序列测定方法,鉴定蜱虫体内的病原体感染情况和蜱虫种类. 结果 从警犬体表及犬舍墙壁共计采集5只饱血蜱和13只饥饿蜱;经PCR鉴定均为血红扇头蜱（Rhipicephalus sanguineus）.蜱虫体内扩增到田鼠巴贝虫（Babesia microti）、伯纳特氏立克次氏体（Goxiella burnetii）、假单胞球菌（Pseu-domonas sp.）、甲基杆菌（Methylobacterium sp.）DNA序列,阳性率分别为27.8％ （5/18）、22.2％ （4/18）、11.1％ （2/18）、11.1％ （2/18）. 结论 南宁某警犬养殖训练基地蜱虫存在一定比例的田鼠巴贝虫、伯纳特氏立克次氏体、假单胞球菌、甲基杆菌感染阳性率,对接触人员、及其他牲畜有潜在感染的风险,应加强预防和控制.Objective To survey the infestation and pathogens infections of ticks at a police dog breed-ing and training base in Guangxi Nanning.Methods In July 2013,ticks from the police dogs and doghouses at a police dog breeding and training base were sampled; Using nest-PCR,PCR,with the universal primers of 18S rRNA of Babesia spp.,16S rRNA of prokaryote,and mitochondria 16S rRNA of eukaryote,the infection of pathogens in ticks was investigated.Results A total of 5 engorged ticks and 13 non-blood sucking ticks were collected.The ticks sampled were Rhipicephalus sanguincus identified by PCR.Some of the ticks from the police dogs and doghouses had positive amplifications of B.microti,C.burnetii,Pseudomonas sp.,Methylobacterium sp.,and the positive rates were 27.8%（5/18）,22.2%（4/18）,11.1%（2/18）,11.1%（2/18）,respectively.Conclusion Ticks at the police dog breeding and training base were found.The ticks were R.sanguineus according to the results of PCR amplification.And some of the tested ticks were infected with B.microti,C.burnetii,Pseudomonas sp.,Methylobacterium sp..Those microbes are zoonosic pathogens and are threatening to human and livestocks.Prevention and control strategy should be strengthened.

关 键 词：血红扇头蜱 犬 巢式PCR 田鼠巴贝虫 立克次氏体 

分 类 号：R563.1[医药卫生—呼吸系统]