激惹诱导心律失常大鼠血清中神经肽与NADPH氧化酶表达效应及中药作用机制  

The Expressive Reduced form Ofnicotinamide-adenine Dinucleotide Phosphate and Neuropeptide Y in Oxidative Stress Injury

作　　者：高跃[1] 董川[2] 姜均文 边振[4] 昌艳艳[3] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]沈阳市第二中医医院,辽宁沈阳110101 [3]辽宁中医药大学附属医院,辽宁沈阳110032 [4]山东省中医药研究院,山东济南250014

出　　处：《中华中医药学刊》2015年第4期922-924,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省科技厅计划项目(2011020236-301)

摘　　要：目的:探讨激惹诱导心律失常大鼠血清中神经肽与NADPH表达效应及中药作用机制。方法:选40只SD大鼠,随机分为模型组A、中药方剂组B、β受体阻滞剂组C、造模前对照D 4组,每组12只。A、B、C组分别与造模前12只大鼠中血清NPY、NADPH氧化酶做比较分析。利用酶联免疫吸附法(ELISA)观察造模前后各组大鼠血清神经肽Y(NPY)和NADPH氧化酶的含量。结果:模型组血清NPY及NADPH氧化酶含量明显高于造模前对照组(P<0.05),中药组NPY、NADPH氧化酶水平较西药组明显降低,有明显差异(P<0.05),有统计学意义。结论:神经肽Y与NADPH氧化酶均与心率失常密切相关。中药可以抑制血清中神经肽水平,降低NADPH氧化酶的含量(P<0.05)。神经肽Y与NADPH氧化酶均与心率失常密切相关。中药方剂组,可明显降低大鼠血清中神经肽含量以及NADPH氧化酶水平含量,中药疏肝解郁定悸方对提高机体抗应激能力有一定作用。Objective： To investigate t The expressive reduced form ofnicotinamide-adenine dinucleotide phosphate oxidase and neuropeptide Y in oxidative stress injury. Methods： 40 SD rats,by the bowel stimulation,were induced fast arrhythmia. Before and after modeling,the plasma neuropeptide Y（ NPY） and（ NADPH） changes and the corresponding changes in each period of ECG were recorded. Changes in serum NPY and NADPH was detected by using ELISA method. Results： The plasma NPY and NADPH were increased significantly（ P〈0. 01）,and this state persisted. ECG was characterized with tachyarrhythmia. Conclusion： Liver controlling dispersion theroy regulating bowel is associated with rapid arrhythmia. The elevation of NPY and NADPH plays an important role in the pathological changes of tachyarrhythmia.

关 键 词：血浆神经肽Y NADPH氧化酶 心律失常 肝主疏泄 

分 类 号：R541[医药卫生—心血管疾病]