檀香形成部位内生真菌多样性研究  

Diversity of Endophytic Fungi in sandalwood forming section of Santalum album

作　　者：孙思胜[1,2] 张大为[1] 孟志霞[1] 郭顺星[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所生物技术中心,北京100193 [2]许昌学院食品与生物工程学院/河南省博士后研发基地,河南省许昌市461000

出　　处：《中国医药生物技术》2015年第2期119-124,共6页Chinese Medicinal Biotechnology

基　　金：国家自然科学基金(31170016)

摘　　要：目的旨在考查檀香形成部位内生真菌的定植情况,并探讨檀香结香部位木材和正常生长的健康木材之间内生真菌的差异,揭示真菌诱导檀香结香的原因。方法采用组织块分离法对檀香结香部位木材和正常生长的健康木材进行内生真菌的分离培养和统计分析,并采用形态学和分子生物学两种方法对分离得到的真菌进行鉴定。结果檀香结香部位木材内生真菌定植率较高(92.5%),而正常木材内生真菌定植率较低(27.5%)。分离率也是檀香结香部位木材较高(0.9),健康正常木材较低(0.25)。檀香正常生长的健康白木材分离的内生真菌只有2种;结香部位木材分离得到13种,隶属于4个属,分别为拟茎点霉属、镰孢属、曲霉属和Phaeoacremonium。在檀香结香部位分离的内生真菌中,优势菌属是镰孢属和拟茎点霉属;在檀香健康木材分离的优势菌属是拟茎点霉属。结论檀香结香部位木材内生真菌表现出了较高的种属多样性。檀香局部结香可能是由外界损伤和真菌的侵染诱导而形成的。Objective To investigate endophytic fungi colonization in sandalwood of Santalum album and the differences in endophytic fungi between sandalwood and normal white healthy wood, which may reveal the cause of sandalwood formation induced by fungi. Methods Tissue block method was used to isolate and statistically analyze the endophytic fungi, which were then identified by morphology and molecular biology methods. Results The colonization rate of endophytic fungi in sandalwood（92.5%） was higher than that in normal white healthy wood（27.5%）. Two fungal strains were recovered from normal white healthy wood, which belonged to two genus respectively. 13 fungal stains were isolated from sandalwood, which belonged to four genus, Phomopsis, Fusarium, Aspergillus and Phaeoacremonium. Conclusion There are diverse endophytic fungi in sandalwood. The most frequently isolated genera are Fusarium and Phomopsisin the sandalwood. While, the most frequently isolated genera is Phomopsis in the normal white healthy wood. External damaging and fungi inducing maybe due to portion sandalwood formation.

关 键 词：檀香 内生真菌 结香部位木材 健康正常木材 

分 类 号：S567.19[农业科学—中草药栽培] Q949.32[农业科学—作物学]