猪囊尾蚴刷状缘结构的冷冻蚀刻研究  

FREEZE-ETCH STUDY ON THE BRUSH BORDER STRUCTURE OF CYSTICERCVS CELLVLOSAE

作　　者：孟宪钦[1] 王松山[1] 周文琴[1] 王伯霞[1] 张玉英[1] 刘贵昇 

机构地区：[1]河北医学院寄生虫学教研室及电镜室

出　　处：《中国寄生虫学与寄生虫病杂志》1991年第2期130-133,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：应用透射电镜观察了猪囊尾蚴经冷冻蚀刻技术制备样品的刷状缘结构,尤其虫体的膜构造及膜内颗粒,并对皮层及焰细胞构造作了详细的描述。头节微毛分为两个部分,粗的微毛基部与细的棘样尖端,微毛基部PF面具有膜内颗粒,棘样尖端的4个膜面均很光滑,未见颗粒。微毛断面观可见微毛纤丝,呈颗粒状,不规则的分布于微毛基质之中。在实质层中靠近肌层处很易见到实质细胞、钙质小体及焰细胞。焰细胞由焰细胞体、焰细胞纤毛束及漏斗样滤管三部分组成。The images of C. cellulosae detected by freeze-itch technique were exactly similar to those observed in the thin section.The scolex microtriches might be divided into two parts, a thick proximal portion and a thinner spinal distal end, the former measuring about 0.2-0.4μm in length, the latter measuring about 1-2μm in length. On the extracellular surface of the proximal portion of microthrix, 1 or 2 particles could occasionally be seen, while numerous particles appeared on the protoplasmic fracture face (PF face) of the proximal portion. The PF face were closely packed and irregularly distributed with membranous particles, which were spherical or subspherical in shape, measured 0.01-0.02μm in diameter. The extracellular fracture face (EF face) of proximal portion and all four membranous faces of spinal end were smooth.The tegumentary plasmic membrane was 0.02μm in thickness, with smooth ES face. The PF face of this membrane packed closely with numerous particles, which were similar with those on the PF face of the microthrix. Numerous globular or discoidal vesicles scattered at random in the tegumentary matrix. Most mitochondria were near the basement membrane of the matrix.In the paranchymal layer, the paranchymal cells, calcareous corpuscles and flame-cells: could easily be seen. Most of the calcareous corpuscles and flame-cells were located closely beneath the muscle layer. The flame-cell of C. cellulosae mainly consisted of three portions,. cell body, a bundle of cilia and a filtered cell lumen. The flame-cell body had a large nucleus and relatively few cytoplasm with several mitochondria. The cilia of flame-cell formed a bunch of cilium tuft bundled up closely by protoplasmic membrane. The diameter of tuft meausred about 2.2-3.0μm. These cilia were arranged regularly in several lines, each line contained 1011- cilia. The total number of the cilia in a flame-cell tuft was 70-90. The cilia appeared as regularly hexagonal near the flame-cell body and cylindrical at the distal end. The cilium was 0.2-0.25μ

关 键 词：猪囊尾蚴 刷状缘结构 冷冻蚀刻 

分 类 号：R383.34[医药卫生—医学寄生虫学]