不同强度耐力训练对衰老小鼠骨骼肌线粒体抗氧化及生物发生相关基因表达的影响  

Benefit of Endurance Training in Reduction of Skeletal Muscle Oxidative Stress of Aged Mice

作　　者：付绍婷 邱守涛[1,2] 李茜[1,2] 许迎迎[1,2] 陈彩珍[1,2] 邱林利[1,2] 卢健[1,2] 

机构地区：[1]华东师范大学体育与健康学院,上海200241 [2]青少年健康评价与运动干预教育部重点实验室

出　　处：《中国运动医学杂志》2014年第11期1069-1074,共6页Chinese Journal of Sports Medicine

基　　金：上海市浦江人才计划资助项目(10PJC029)

摘　　要：目的:探讨不同强度耐力训练对衰老小鼠骨骼肌线粒体抗氧化及生物发生相关基因表达的影响。方法:本文以3月龄快速老化小鼠(senescence accelerated mouse prone 6,SAMP6)为衰老模型,8周跑台运动为运动干预手段,将小鼠随机分为快速衰老对照组(P6)、高强度运动组(H)、中强度运动组(M)、低强度运动组(L),并以SAMR1(senescence accelerated mouse resistant 1)小鼠为正常对照组(R1)。采用RT-PCR方法检测各组小鼠骨骼肌线粒体MnSOD、GPx、PRDX3和PRDX5抗氧化相关基因以及PGC-1α、NRF-1、T-fam生物发生相关基因mRNA表达水平。结果:与R1组相比,P6组雌、雄性小鼠PGC-1α、MnSOD、GPX以及雄性小鼠NRF-1、T-fam、PRDX3和PRDX5 mRNA表达水平显著升高;与P6组相比,8周高、中、低强度耐力训练显著降低雌、雄性小鼠GPX以及雌性小鼠PGC-1αmRNA表达水平,且高强度运动组效果最为明显;高、中强度耐力训练显著降低雄性小鼠MnSOD、PRDX3和PRDX5 mRNA的表达水平,而只有高强度耐力训练显著降低雄性小鼠NRF-1、T-fam mRNA表达水平;与雄性小鼠相比,P6组雌性小鼠GPx、PRDX5 mRNA表达水平显著升高。结论:SAMP6小鼠衰老进程中氧化应激和ROS信号可能刺激线粒体抗氧化和生物发生相关基因转录水平上调。不同强度耐力运动增加了骨骼肌的氧化应激,氧信号刺激了线粒体抗氧化酶和线粒体生物发生。8周不同强度耐力训练可能使机体抗氧化酶和线粒体生物发生产生适应性变化,已适应强度的运动所引起的氧化应激不足以使抗氧化酶蛋白基因和线粒体生物发生相关基因在转录水平上上调,而出现下调现象,这是骨骼肌对耐力训练产生的一种适应性变化,有助于减缓衰老骨骼肌的氧化应激。Objective To investigate the effects of endurance training with different intensities on the gene expression of mitochondria in skeletal muscle related to antioxidation and biogenesis in aged mice.Method 3 months old rapid ageing SAMP6 mice were used as the aging model.They were randomly divided into sedentary control group（P6）,high intensity exercise group（H）,moderate intensity exercise group（M）,and low intensity exercise group（L）.Rapid ageing mice resistant 1 were deployed as normal control group（R1）.Mice in exercise groups were forced to perform 8-week treadmill exercise.Antioxidative gene MnSOD,GPx,PRDX3,and PRDX5,and mitochondrial biogenesis gene PGC-α/NRF-1/T-fam in gastrocnemius were measured by Real-Time PCR.Results The mRNA PGC-α,MnSOD,and GPx levels of male and female mice,and NRF-1,T-fam,PRDX3,and PRDX5 levels of male mice in group P6 increased significandy as compared to group R1.As compared to group P6,the GPX mRNA expression level of male and female mice,and PGC-lamRNA expression level of male mice in groups H,M,and L reduced significantly after the 8-week training,especially in group H.The MnSOD,PRDX3 and PRDX5 mRNA expression levels of male mice in groups H and M reduced significantly,and the NRF-1 and T-fam mRNA expression levels of male mice decreased significantly only in group H.Conclusion In the aging process,the mRNA expression level of mitochondrial antioxidation and biogenesis-related gene in rapid aging mice increases to a certain degree after 8-week treadmill exercise,helping reducing the skeletal muscle oxidative stress.

关 键 词：耐力训练 骨骼肌 线粒体 抗氧化 生物发生 

分 类 号：R87[医药卫生—运动医学]