络瘀通胶囊对大鼠脑缺血-再灌注损伤的作用  

Effect of Luoyutong capsule on focal cerebral ischemia-reperfusion injury in rats

作　　者：王丽晔[1] 赵海苹[2] 王荣亮[2] 张陈诚[2] 刘小蒙[2] 王宁群[2] 罗玉敏[2] 陈志刚[1] 

机构地区：[1]北京中医药大学东方医院脑一科,100078 [2]首都医科大学宣武医院脑血管病研究室北京市老年病医疗研究中心

出　　处：《中国脑血管病杂志》2014年第12期650-655,共6页Chinese Journal of Cerebrovascular Diseases

基　　金：北京市中医药科技项目(JJ2012-07);首都医科大学中医药研究专项(13ZY10)

摘　　要：目的观察并探讨络瘀通胶囊对大鼠脑缺血-再灌注后神经功能的影响及其保护机制。方法采用改良Longa法制备大鼠大脑中动脉阻塞(MCAO)模型,缺血1.5 h后进行再灌注。选取10只大鼠作为假手术组,将40只造模成功的雄性SD大鼠按照随机数字表法分为4组,每组10只:模型组、络瘀通中剂量组(LYTM组)、络瘀通高剂量组(LYTH组)及胞磷胆碱钠组(CS组)。分别于造模后3 d及7 d采用12分评分及前肢踩空实验评价大鼠神经功能,应用蛋白免疫印迹法(Western blot)检测造模3 d后大鼠缺血侧及假手术组同侧脑组织中脑源性神经营养因子(BDNF)、碱性成纤维生长因子(b-FGF)及磷酸化蛋白激酶/蛋白激酶(p-AKT/AKT)的表达,及造模后7 d同侧脑组织中Caspase-12的表达并进行组间比较。结果 (1)神经功能评分:造模后3 d各组12分评分及前肢踩空实验评分差异均无统计学意义(均P>0.05);造模后7 d,LYTM组、LYTH组、CS组较模型组均明显改善(均P<0.05)。(2)Western blot结果显示:1模型组BDNF及b-FGF表达明显少于假手术组(均P<0.05);与模型组比较,LYTM组、LYTH组、CS组均可上调BDNF和b-FGF表达,以LYTH组作用最明显(P<0.01)。2与假手术组比较,模型组p-AKT/AKT表达明显减少(P<0.05);与模型组比较,LYTM组、LYTH组、CS组p-AKT/AKT表达增加,以LYTH组和CS组明显,差异均有统计学意义(均P<0.05)。3在Caspase-12的表达中,模型组裂解后Caspase-12表达较假手术组明显升高,差异有统计学意义(P<0.05)。与模型组比较,LYTM组、LYTH组Caspase-12前体(pro Caspase-12)和裂解后Caspase-12表达有减少趋势,但差异无统计学意义(均P>0.05);CS组Caspase-12前体和裂解后Caspase-12表达水平明显低于模型组(P<0.05)。结论络瘀通胶囊可通过促进神经元存活及再生对大鼠脑缺血-再灌注性损伤起到保护作用,且该保护作用也可能与抑制神经细胞凋亡有关。Objectives To observe the effect of Luoyutong capsule on neurological function following focal cerebral ischemia-reperfusion in rats and to preliminarily study the protective mechanism of Luoyutong capsule for focal cerebral ischemia-reperfusion in rats. Methods A rat model of middle cerebral artery occlusion （MCAO）was induced by the modified Longa method. After 1. 5 h of ischemia,reperfusion started. Ten male SD rats were selected as sham operation group,and forty male SD rats were randomly divided into 4 groups：Model （MCAO）,Luoyutong moderate-dose （LYTM）,Luoyutong high-dose （LYTH）,and citicoline sodium （CS）groups （n=10 in each group）. At day 3 and 7 after modeling,the neurological function of the rats was evaluated by using 12 neurological score and forelimb placing test.＆amp;nbsp;Western blotting was used to detect the expression levels of brain derived neurotrophic factor （BDNF）,basic fibroblast growth factor （b-FGF）,and phosphor/protein kinase （p-AKT/AKT）on the ischemic side of the rats and in the ipsilateral brain tissue at day 3 after modeling,as well as the expression level of Caspase-12 at day 7 after modeling in the ipsilateral brain tissue,and a comparison was performed among the groups. Results （1 ）Neurological score：At day 3 after modeling,there was no significant difference between the 12 neurological score and the forelimb placing test score （all P〉0. 05）;At day 7 after modeling, there were obvious improvement in the LYTM,LYTH,and CS groups compared with model group （all P〈0.05）. （2）The results of western blot showed that①compare with the sham operation group,the expression levels of BDNF and b-FGF were reduced obviously （all P〈0.05）;compare with the MCAO group,the expression levels of the LYTM,LYTH and CS groups could be up-regulated,particularly in the LYTH group （P〈0. 01）;② compare with the sham operation group,the expression level of p-AKT/AKT in MCAO group was decreased obviously （P〈0. 05）;compare with the MCAO

关 键 词：再灌注损伤 神经再生 细胞凋亡 大鼠 脑缺血-再灌注 络瘀通胶囊 

分 类 号：R285.5[医药卫生—中药学]