调节性T细胞、辅助T细胞在慢性阻塞性肺疾病患者肺功能降低中的作用  

Role of Treg,Th cells reduced lung function in patients with chronic obstructive pulmonary disease

作　　者：王成阳[1] 刘向国[2] 彭青和[2] 李达[2] 季乔雪[2] 王传博[3] 李泽庚 

机构地区：[1]安徽医科大学第一附属医院中医科,安徽合肥230022 [2]安徽中医药大学 [3]安徽医科大学第二附属医院 [4]安徽中医药大学第一附属医院

出　　处：《中国老年学杂志》2014年第22期6247-6249,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81072781)

摘　　要：目的探讨调节性T细胞(Treg)、辅助T细胞(Th)在慢性阻塞性肺疾病(COPD)患者肺功能降低中的作用。方法采用肺功能仪观察COPD患者肺功能变化,采用酶联免疫吸附法检测血清γ-干扰素(IFN)、白介素(IL)-4、IL-17变化,采用流式细胞术检测外周血Treg表达。结果与正常组比较,COPD患者肺功能参数降低,6 min步行距离缩短,血清IL-4、外周血CD4+CD25+FOXP3+Treg降低,而血清IFN-γ、IL-17升高(P<0.05或P<0.01)。相关分析结果显示,COPD患者Treg与IL-17呈负相关,IL-17、IFN-γ与IL-4呈负相关;Treg与IL-4呈正相关,IFN-γ与Th1/Th2、IL-17呈正相关;最大呼气流量(PEF)与IL-17呈负相关,FEV1/FVC与IFN-γ、Th1/Th2呈负相关;FEV1与IL-4、Treg呈正相关,FEV1/FVC与Treg呈正相关(P<0.05或P<0.01)。结论 COPD患者Treg表达降低,免疫抑制功能明显下降,失去对炎性细胞的抑制作用,引起Th1/Th2失衡或Th17细胞过表达,导致COPD发生和肺功能降低。Objective To investigate the mechanism of regulatory T cells （Treg）,Helper T cell （Th） in patients of chronic obstruc-tive pulmonary disease （ COPD ） with reduced lung function .Methods Pulmonary function was detected by spirometer in patients with COPD .γ-interferon （ IFN-γ） ,interleukin （ IL）-4 and IL-17 in serum were detected by enzyme-linked immunosorbent assay .Treg of peripheral blood was detected by flow cytometry .Results Compared with those of normal group ,lung function parameters ,6 minutes walking distance , the levels of IL-4 and CD4＋CD25＋FOXP3＋Treg were decreased ,while the expression of IFN-γ,IL-17 in serum were increased in COPD pa-tients（P〈0.05 or P〈0.01）.Treg was negatively correlated with IL-17,and IL-17,IFN-γwere negatively correlated with IL-4.Treg was posi-tively correlated with IL-4, and IFN-γ, Th1/Th2 were positively correlated with IL-17.Pulmonary function parameters , such as PEF and FEV1/FVC,were negatively correlated with IFN-γ,Th1/Th2 and IL-17.FEV1 was positively correlated with IL-4,Treg（ P〈0.01 or P〈0. 05）.Conclusions The expression of Treg is decreased significantly in COPD patients ,and immune suppression is decreased .Treg can loss the function of inhibition to inflammatory cells which could cause imbalance of Th 1/Th2 or over-expression of Th17 cells,and result to COPD and reduce pulmonary function .

关 键 词：慢性阻塞性肺疾病 肺功能 调节性T细胞 辅助T细胞 

分 类 号：R563[医药卫生—呼吸系统]