左、右归丸通过TGF-β1/Smad4信号途径对大鼠骨髓间充质干细胞成骨诱导的影响  

The effect of Zuo Gui Wan and You Gui Wan induce bone marrow mesenchymal stem cells to osteoblast through TGF-β1 / Smad4 signaling pathway

作　　者：何丽娟[1] 宋囡[1] 何文智[2] 孙月娇 初杰[3] 任艳玲[1] 

机构地区：[1]辽宁中医药大学基础医学院,辽宁沈阳110847 [2]辽宁中医药大学药学院 [3]辽宁中医药大学方剂学科

出　　处：《中国老年学杂志》2014年第22期6382-6384,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(81373527);辽宁省自然科学基金项目(201102148)

摘　　要：目的研究左、右归丸含药血清经转化生长因子(TGF)-β1/Smad4信号途径对骨髓间充质干细胞(BMSCs)成骨诱导的影响。方法以左归丸、右归丸水煎液和阳性对照药补佳乐、蒸馏水给SD大鼠灌胃后取血制备含药血清(含有10%含药血清的诱导剂),与诱导剂组共5种处理因素对BMSCs进行干预,选用Western印迹法检测碱性磷酸酶(ALP)蛋白的表达,用免疫组化SP法检测TGF-β1和Smad4蛋白的表达。结果左、右归丸与空白组和诱导剂组比可显著促进ALP蛋白的表达,增强TGF-β1和Smad4蛋白的表达(P<0.01),左归丸组与右归丸组比较可上调ALP、Smad4蛋白的表达(P<0.01)。结论左归丸与右归丸可能通过增强ALP、TGF-β1、Smad4蛋白的表达,影响TGF-β1/Smad4信号途径,从而协同诱导剂促进BMSCs的成骨诱导,并且左归丸较右归丸促进作用更明显。Objective To observe the effects of serum containing ZuoGuiWan and Youguiwan on the osteogenic induction in bone marrow mesenchymal stem cells （ BMSCs） through transforming growth factor （ TGF）-β1/Smad signaling pathway .Methods Containing se-rum of Zuoguiwan,Youguiwan,positive control drug （Estradiol Valerate,BJL）and distilled water were prepared to separately add inducer , with inducer group,simultaneously intervene the BMSCs.The expression of alkaline phosphatase （ALP） protein was determined by Western blot,the expression of TGF-β1and Smad4 protein were tested by immunohistochemical streptavidin-perosidase method.Results Compared with that of control and inducer groups ,Zuiguiwan and Youguiwan remarkablely promoted the expression of ALP protein ,upregulated the ex-pressions of TGF-β1 and Smad4 protein （P〈0.05）.Compared with Youguiwan group ,Zuoguiwan group enhanced the expressions of ALP and Smad4 protein（P〈0.05）.Conclusions Through ALP enhancement the expressions of TGF-β1 and Smad4 protein,Zuiguiwan and Yougui-wan could effect TGF-β1/Smad4 signaling pathways ,thus cooperate with inducer on the osteogenic induction in BMSCs .

关 键 词：左归丸 右归丸 骨髓间充质干细胞 碱性磷酸酶 转化生长因子β1 SMAD4 

分 类 号：R285.5[医药卫生—中药学]