Cthrc1及TGF-β1/Smad信号通路在硬皮病成纤维细胞中的表达及其意义  

The Expression and Significance of Cthrc1 and TGF-β1/Smad Signaling Pathway in Scleroderma Fibroblasts

作　　者：张丽霞[1] 周竹[2] 王倩[1] 卢葳[1] 陈金[1] 

机构地区：[1]四川省医学科学院四川省人民医院皮肤性病研究所,四川成都610072 [2]四川省成都市双流县第一人民医院皮肤科,四川双流610200

出　　处：《中国皮肤性病学杂志》2015年第4期345-348,共4页The Chinese Journal of Dermatovenereology

基　　金：四川省卫生厅科研课题基金项目(110226)

摘　　要：目的初步探讨胶原三螺旋重复蛋白1(Cthrc1)及TGF-β1/Smad信号在硬皮病发病机制中的作用。方法采用免疫组织化学技术检测30例硬皮病皮损组织及30例正常皮肤组织成纤维细胞中Cthrc1,TGF-β1,Smad2,Smad3,Smad7的表达情况。结果硬皮病皮损组织中Cthrc1,TGF-β1,Smad2,Smad3,Smad7的表达(6 620.40±1 758.34,1 098.97±441.08,24 008.58±7 431.01,54 983.22±17 741.32,1 098.34±332.00)均较正常皮肤组织(856.14±169.49,221.51±58.76,1 816.42±313.63,2 069.49±586.66,132.61±66.26)显著升高,差异均有统计学意义(P<0.05);在硬皮病皮损组织中TGF-β1与Cthrc1,Smad2,Smad3,Smad7的表达呈显著正相关性(P<0.05)。结论硬皮病皮损组织成纤维细胞中Cthrc1的异常表达及TGF-β1/Smad信号通路异常活化存在一定的相互作用,并可能共同参与了硬皮病皮肤胶原纤维进行性硬化的过程。Objective To explore the role of Cthrcl and TGF-β1/Smad signaling pathways in the pathogenesis of scler- oderma. Methods The expression of Cthrcl, TGF-β1, Smad2, Smad3 and Smad7 in 30 cases of scleroderma skin lesions and 30 cases of normal skin fibroblasts was tested by immunohistchemical technique. Results The expressions of Cthrcl, TGF-131, Smad2, Smad3 and Smad7 in scleroderma （ 6 620.40 ± 1 758. 34, 1 098.97 ±441.08,24 008.58 ± 7 431. 01,54 983.22 ± 17 741.32,1 098.34 ± 332.00） were significantly higher than those in the control group （ 856.14 ± 169.49,221.51 ± 58.76,1 816.42 ± 313.63,2 069.49 ± 586.66,132.61 ± 66.26 ;P 〈 0.05） ;The expression of TGF-β1 had positive correlation with that of Cthrcl, Smad2, Smad3 and Smad7 ratios of integral density （ IOD ） （ P 〈 0.05 ）. Conclusion There is interaction between abnormal expression of Cthrcl and abnormal activation of TGF-β1/Smad signaling pathways in sclero- derma fibroblasts which may participate in the process of scleroderma skin collagen fibers sclerosis.

关 键 词：硬皮病 胶原三螺旋重复蛋白1 TGF-Β1 SMAD2 SMAD3 SMAD7 

分 类 号：R593.25[医药卫生—内科学]