不同年龄牦牛睾丸蛋白基因产物9.5和神经肽Y的分布比较  

Distribution of protein gene product 9.5 and neuropeptide Y in yak testis at different ages

作　　者：袁莉刚[1] 曲亚玲[1] 谷来凤[1] 田旦增 

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《解剖学报》2015年第2期232-237,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(31160488);甘肃省自然科学基金资助项目(1010RJZA210);甘肃省教育厅科技资助项目(0902B-03)

摘　　要：目的探讨蛋白基因产物9.5（PGP 9.5）和神经肽Y（NP-Y）在不同年龄牦牛睾丸的分布特征。方法采取睾丸摘除术收集3～4月龄睾丸9对,3、4岁牦牛睾丸10对,10～12岁牦牛睾丸7对,常规石蜡包埋、切片,免疫组织化学SP法观察PGP 9.5和NP-Y在不同年龄牦牛睾丸的分布特点。结果 PGP 9.5和NP-Y显著分布于幼龄及青年牦牛睾丸Leydig细胞及间质血管周围或血管壁内,两者在青年牦牛睾丸初级精母细胞为阴性,其余生精细胞为阳性;且阳性信号在睾丸体最强,其次为头部,尾部最弱;各年龄段Sertoli细胞中NP-Y为阳性表达,PGP 9.5均为阴性;老龄牦牛睾丸血管PGP 9.5阳性信号明显,而NP-Y基本无表达。结论青年牦牛睾丸组织PGP 9.5及NP-Y的显著分布对睾丸生殖功能调节发挥着重要的调控作用;提示NP-Y在老龄牦牛睾丸血管的分布显著降低。Objective To investigate the distribution of gene product 9. 5 and neuropeptide Y（ NP-Y） in yak testis and in an attempt of exploring their relationships with testicular spermatogenesis at different ages. Methods Using immunohistochemical SP technique,we depicted the distribution characteristics of PGP 9. 5 and NP-Y in yak testis： 9 pairs of yak calves in age from 3 to 4 months,10 pairs of young adult yak from 3 to 4 years and 7 pairs of aging yak from 9 to 13 years old were prepared by orchiectomy surgery,bedded in paraffin and sectioned serially. Results NP-Y and PGP 9. 5immunoreactivities were abundantly distributed in gonads,mainly associated with Leydig cell nests,blood vessels,and seminiferous tubules. PGP 9. 5 and NP-Y were mainly expressed in spermatogonia of yak calves testis and in spermatogenic cells of young yak testis. By contrast,no NP-Y and PGP 9. 5 immunoreactivities were observed within the primary spermatocytes. The expression of PGP 9. 5 and NP-Y in corpus testis were stronger than the caput and cauda of the testis.A small number of PGP 9. 5-immunoreactive cells was detected in testis vscellum of aging yak. Conclusion Our results suggest that the PGP 9. 5 and NP-Y expression is significantly different in young adult yak testis and may serve as regulators for testicular functions. The significant dcrease of distribution of NP-Y in the wall of vascular structure in aging yak testis.

关 键 词：蛋白基因产物9.5 神经肽Y 睾丸 年龄 免疫组织化学 牦牛 

分 类 号：S852.162[农业科学—基础兽医学]