氯离子通道-6基因位点rs3737964单核苷酸多态性与冠心病的关系  

Association between polymorphism of chloride channel-6 rs3737964 gene and coronary heart disease

作　　者：张莉[1] 张涛[2] 程龙献[3] 

机构地区：[1]湖北省肿瘤医院ICU,武汉430079 [2]湖北省妇幼保健院超声科 [3]华中科技大学同济医学院附属协和医院心内科

出　　处：《临床心血管病杂志》2015年第3期267-272,共6页Journal of Clinical Cardiology

摘　　要：目的：探讨氯离子通道-6基因位点rs3737964单核苷酸多态性与冠心病发生的关系。方法：本研究收集冠心病患者1 193例（冠心病组）和非冠心病1 200例（对照组）。采用修订后的Inter-hearnt调查表进行流行病学调查,应用实时荧光定量多聚酶链反应技术之TaqMan探针法检测所有研究对象氯离子通道-6基因rs3737964位点多态性。结果：研究对象中氯离子通道-6基因多态性位点rs3737964的T和C等位基因的频率分别是10.4%和89.6%,各基因型分布符合Hardy-Weinberg平衡定律（P=0.178）。rs3737964位点基因型TT频率分布在冠心病组（2.3%）明显高于对照组（1.0%）,与基因型为CC者相比,基因型为TT者其患冠心病的危险性增加（OR=2.318,95%CI=1.168~4.063,P=0.016 3）,调整混杂因素后差异仍有统计学意义（OR=2.925,95%CI=1.287~6.65,P=0.010 4）。分层分析结果显示：rs3737964位点的TT基因型在60岁以上人群中患冠心病的危险性明显增加（调整混杂因素后OR=3.597,95%CI=1.191~10.858,P=0.023 2）。在男性人群中TT型基因型患冠心病的危险性明显增加（OR=3.669,95%CI=1.401~9.608,P=0.008 1）。在吸烟的人群中TT基因型者患冠心病的危险性明显增加,调整混杂因素后差异仍有统计学意义（OR=3.187,95%CI=1.038~9.787,P=0.042 9）。结论：氯离子通道-6基因位点rs3737964多态性可能是罹患冠心病的遗传易感性基因之一,〉60岁、吸烟及男性人群中TT基因型者更易患冠心病。Objective：To explore the relationship between single nucleotide polymorphism of rs3737964 of chloride channel-6（CLC-6）gene and coronary heart disease（CHD）in Chinese.Method：One thousand one hundred and ninety-three CHD patients and 1 200 control subjects were collected.The epidemiology datum was gathered by Inter-heart questionnaire interview.Then the CLC-6rs3737964 polymorphism of extracted deoxyribonucleic acid（DNA）was genotyped by real-time quantitative polymerase chain reaction（real-time Q-PCR）method.Result：Frequencies of T and C alleles of chloride channel-6rs3737964 were 10.4%and 89.6%in the all investigated population respectively,the observed and expected genotype was in agreement with the predicted Hardy-Weinberg equilibrium values（P=0.178）。The frequencies of TT genotype of rs3737964 were significantly higher in the CHD group（2.3%）than the control group（1.0%）.The TT genotype had a significantly increased risk of CHD（OR=2.925,95%CI=1.168-4.063,P=0.0163）compared with the CC genotype,this increased risk was still significant when adjusted for the confounding factors（OR=2.925,95%CI=1.287-6.65,P=0.0104）.The population which beyond 60 years old whose OR=3.597,95% CI=1.191-10.858,P=0.0232 after adjusted the confounding factors.The increased risk was significantly higher in TT genotype of male after adjusted（OR=3.669,95%CI=1.401-9.608,P=0.0081）.The increased risk was significant in TT genotype who smoking when adjusted the confounding factors,OR=3.187,95%CI=1.038-9.787,P=0.0429.Conclusion：The result suggest that the chloride channel-6rs3737964 polymorphism may be associated with the CHD,especially with the TT genotype in age who beyond 60 years old,smoking and male.

关 键 词：氯离子通道-6 单核苷酸多态性 冠心病 

分 类 号：R541.4[医药卫生—心血管疾病]