沉默LAIR-1表达对卵巢癌细胞增殖功能的影响  

In vitro study on the effect of silencing LAIR-1 on proliferation of ovarian cancer cells

作　　者：何晓丽[1] 付爱丽[2] 曹奇志[1] 于文征[3] 薛江楠[1] 

机构地区：[1]滨州医学院免疫学教研室,烟台264003 [2]中国人民解放军第107医院检验科,烟台264003 [3]滨州医学院附属医院血液科,滨州256600

出　　处：《现代免疫学》2015年第2期119-123,共5页Current Immunology

基　　金：国家自然科学基金(81273200);山东省自然科学基金(ZR2010HM072);山东省高校科技计划资助项目(J08LG04)

摘　　要：沉默白细胞相关免疫球蛋白样受体1(LAIR-1)在卵巢癌H08910细胞的表达,探讨其对卵巢癌细胞增殖功能的影响。构建LAIR-1的shRNA慢病毒表达载体,转染人浆液性卵巢癌细胞系H08910。RT-PCR和Western blot检测H08910细胞中LAIR-1分子的沉默效率;采用PI染色法和MTT法检测沉默LAIR-1表达对HO8910细胞周期及增殖功能的影响。成功建立沉默LAIR-1分子表达的稳定HO8910细胞株。与阴性对照组比较,转染LAIR-1-shRNA慢病毒载体的HO8910细胞中LAIR-1的mRNA及蛋白水平表达均明显降低(P<0.05)。LAIR-1-shRNA组G1期细胞数目明显低于CON组和NCshRNA组(P<0.05);沉默LAIR-1分子表达后,LAIR-1-shRNA HO8910细胞的增殖率明显高于NC-shRNA HO8910组和空白对照组(P<0.05)。沉默卵巢癌细胞HO8910LAIR-1分子表达可明显增强细胞增殖的能力,提示卵巢癌细胞表达的LAIR-1可能对癌细胞的生物学功能发挥抑制作用。To investigate the effect of leukocyte-associated immunoglobulin like-receptor 1（LAIR-1）on the proliferation of ovarian cancer cells HO8910 through silencing the expression of LAIR-1.Lentivirus-mediated short hairpin RNA（shRNA）target,HO8910 cells were infected by LAIR-1.Real-time PCR and Western blotting were used to analyze the influence of lentivirus-mediated shRNA infection on the expression of LAIR-1mRNA and protein in HO8910 cells.Then,PI staining and MTT assay were performed to determine the effect of silencing of LAIR-1on cell cycle and proliferation of HO8910 cells.A stable LAIR-1knockdown HO8910 cell line was successfully generated.Transfection of H08910 cells with LAIR-1-shRNA lentiviral vector resulted in a significantly decreased expression of LAIR-1at both mRNA and protein levels（P〈0.05）.LAIR-1knockdown in HO8910 cells resulted in a significant decrease in the proportion of cells at G1phase（P〈0.05）.Inhibition of LAIR-1expression by lentivirus-delivered shRNA in HO8910 cells significantly promoted cell proliferation.（P〈0.05）.The results indicates that silencing of LAIR-1expression in ovarian cancer cell line HO8910 significantly promotes cell proliferation,and LAIR-1maybe play a negative role in ovarian cell carcinogenesis.

关 键 词：LAIR-1 卵巢癌 SHRNA 细胞周期 增殖 

分 类 号：R392.12[医药卫生—免疫学]