成年期追赶生长大鼠体成分变化特征及其与机体胰岛素敏感性的关系  

The characteristics of changes in body composition in adult catch-up growth rat and its association with system insulin sensitivity

作　　者：陈璐璐[1] 李燕[1] 余珊[1] 鲁晓丹[1] 胡祥[1] 郑涓[1] 邓秀玲[1] 黎慧清[1] 张皎月[1] 夏文芳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内分泌科,武汉430022

出　　处：《中华内分泌代谢杂志》2015年第3期257-262,共6页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（81170782,81471069）

摘　　要：目的观察成年期追赶生长（CUGA）大鼠体成分的变化特征，探讨其与机体胰岛素敏感性的关系。方法sD大鼠以随机数字表法随机分为10组，其中5组为普通饮食组（NC），普通饮食喂养4、5、6、8、12周；另外5组为追赶生长组（RN），热卡限制4周后恢复普通饮食0、1、2、4、8周。实验结束后检测体成分和高胰岛素一正葡萄糖钳夹试验的葡萄糖输注率（GIR。如）。结果热卡限制期末，RN组全身脂肪含量（BFM％）、腹部脂肪含量（AFM％）、腹部脂肪比率和脂肪瘦组织比率均明显低于NC组（P〈O．05）；而瘦组织含量（LBM％）相对增加（P〉0．05）。饮食恢复期，RN组上述体脂指标逐渐增加，而LBM％相对下降。RN组GIR60-120与BFM％、AFM％和腹部脂肪比率显著负相关（P〈0．01）。其中，腹部脂肪比率对GIR60-120的影响大于BFM％和AFM％。结论CUGA实质是与瘦组织增长不成比例的脂肪优先追赶和脂肪重新分布过程。这种追赶性体脂增加尤其是体脂再分布可能是决定CUGA机体胰岛素敏感性的重要因素。Objective To observe dynamic changes in body composition in adult catch-up growth （CUGA） rat model and to explore the relationship between body composition and system insulin sensitivity in CUGA. Methods Sprague-Dawley rats were randomly divided into normal chow （ NC ） and catch-up growth with normal chow （ RN ） groups. Body composition was measured by dual-energy X-ray absorptiometry, and average glucose infusion rate 60-120 （GIR60-120） in hyperinsulinemic-euglycemic clamp was calculated to evaluate systemic insulin sensitivity. Results After 4-week calorie restriction, percentage of body fat mass （ BFM% ）, percentage of abdominal fat mass （ AFM% ） and AFM to BFM ratio were lower in RN group than NC group（ P〈0.05 ）, while percentage of lean body mass （ LBM% ） was relative higher in RN group （ P〉0.05 ）. After refeeding, BFM% , AFM%, and AFM to BFM ratio in RN group were all increased with the time, whereas LBM% was gradually decreased. In RN group, GIR60-120 was positively correlated with BFM% , AFM% , and AFM to BFM ratio （ P〈0.01 ）, but there was no linear relationship between GIR60-120 and LBM%. Among these factors, AFM to BFM ratio exhibited more significant relationship with GIR60-120 than BFM% and AFM%. Conclusions The essence of CUGA is a progress that is characterized by a disproportionately higher rate of body fat than lean tissue gain and a redistribution of body fat. This preferential catchup fat, especially the redistribution of body fat, may be a critical determinant for system insulin sensitivity in CUGA.

关 键 词：追赶生长 胰岛素抵抗 脂肪 瘦组织 体成分 

分 类 号：R587.1[医药卫生—内分泌]