响应面法优化海藻酸盐复合凝胶微球的制备工艺  

Process Optimization for the Preparation of Alginate Composite Microgel Beads by Response Surface Methodology

作　　者：陈秀琼[1] 颜慧琼[1] 李嘉诚[2] 冯玉红[2] 史载锋[1] 王向辉[1] 林强[1] 

机构地区：[1]海南师范大学化学与化工学院,海南海口571100 [2]海南大学材料与化工学院,海南海口570228

出　　处：《精细化工》2015年第4期392-398,共7页Fine Chemicals

基　　金："十二五"国家科技支撑计划资助项目(2011BAE06A06-7;2011BAEO6B04-7);海南省重点科技计划资助项目(ZDXM20120003);海口市重点科技计划项目(2011-0104)~~

摘　　要：以氯化钙（Ca Cl2）和壳聚糖（CS）作为交联体系,膨润土（BT）作为吸附剂制备了负载甲氨基阿维菌素苯甲酸盐的海藻酸盐（SA）复合凝胶微球,以其载药率（DLR）和平衡溶胀率（ESR）的比值作为响应值（UR,UR=DLR/ESR）,采用Box-Behnken设计建立模型和考察海藻酸钠质量分数（1.00%～3.00%）、膨润土质量分数（1.00%～5.00%）、氯化钙浓度（0.10～0.40 mol/L）和壳聚糖质量分数（0.50%～1.50%）对响应值的影响。结果表明,响应值与四因素关系符合二次模型,在实验范围内,该数学回归模型具有良好的预测性。在各因素设定范围内预测最佳工艺条件为：海藻酸钠质量分数2.39%、膨润土质量分数2.81%、氯化钙浓度0.24 mol/L、壳聚糖质量分数0.71%。在该条件下进行3次重复实验,实际测得的平均响应值为3.550 9%,与理论预测值3.583 6%无显著性差异。在该条件下制备的复合凝胶微球,包封率为98.31%,载药率为2.11%,并且具有良好的缓释性能。The alginate（SA） composite microgel beads loaded with the emamectin benzoate were prepared with calcium chloride（Ca Cl2） and chitosan（CS） as cross-linking agents and bentonite（BT）as an adsorbent.Furthermore,the Box-Behnken design was used to build the model and to analyze the effects of the mass fraction of sodium alginate（1.00% ～ 3.00%）,the mass fraction of bentonite（1.00% ～ 5.00%）,the concentration of calcium chloride（0.10 ～ 0.40 mol/L） and the fraction concentration of chitosan（0.50% ～ 1.50%） on the response value,regarding the ratio of drug loading rate（DLR） to equilibrium swelling ratio（ESR） as the response value（UR,UR = DLR/ESR）.The experimental results show that the relationship between the response value and four independent variables were in line with the quadratic model.The mathematical regression model had good predictability and the optimal conditions within the experimental ranges for the response value were forecasted to be 2.39%sodium alginate,2.81% bentonite,0.24 mol/L calcium chloride and 0.71% chitosan.Under these optimal conditions,the average value of the actual response value in the three replicated experiments is3.550 9%,which is not significantly different from the value of 3.583 6% predicted by the model.In addition,the composite beads prepared under the optimal conditions have an encapsulation efficiency of2.11%,a drug loading rate of 98.31% and good sustained-release properties.

关 键 词：海藻酸盐 优化 响应面法 复合凝胶微球 载药率 平衡溶胀率 缓释性能 功能材料 

分 类 号：TQ450.68[化学工程—农药化工]