高效液相色谱法同时测定鱼腥草中绿原酸和金丝桃苷含量  

Simultaneous Determination of Chlorogenic Acid in Herba Houttuyniae and Hyperin HPLC

作　　者：李永伟[1] 何兵[2] 周德兵[1] 蒋达坤 

机构地区：[1]四川省安岳县人民医院,四川资阳642350 [2]泸州医学院药物研究中心,四川泸州646000

出　　处：《中国药业》2015年第7期49-51,共3页China Pharmaceuticals

摘　　要：目的建立同时测定鱼腥草中绿原酸和金丝桃苷含量的高效液相色谱法。方法色谱柱为Akzo Nobel Kromasil C18柱（250mm×4.6mm,5μm）,流动相为乙腈-0.1%磷酸溶液,梯度洗脱,检测波长326nm。结果绿原酸和金丝桃苷进样量在0.204-2.040μg（r=0.999 9）和0.210-2.100μg（r=0.999 8）范围内与峰面积呈良好线性关系,平均回收率分别为98.46%（RSD=1.00%）和98.37%（RSD=0.97%）。结论该方法简便、快速、准确、重复性好,可用于鱼腥草中2种有机酸类成分的含量测定。Objective To establish a HPLC method for the simultaneous determination of chlorogenic acid and hyperin contents in Herba Houttuyniae.Methods The separation was carried out on the Akzo Nobel Kromasil C18 column（250mm ×4.6mm,5μm）.The mobile phase was methanol-0.1% phosphoric acid with gradient elution.The detective wavelength was set at 326nm.Results Chlorogenic acid and hyperin had good linearity in the range of 0.204-2.040 μg（r=0.999 9） and 0.210-2.100μg（r=0.999 8） respectively.The average recovery rates were 98.46%（RSD =1.00%） and 98.37%（RSD =0.97%） respectively.Conclusion This method is sim ple,rapid and accurate with good repeatability,and can be used for quantitatively determining the contents of 2 kinds of organic acid component in Herba Houttuyniae

关 键 词：高效液相色谱法 鱼腥草 绿原酸 金丝桃苷 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]