新型心肌梗死大鼠模型制备方法的建立和模型鉴定  

Establishment and assessment of rat model of myocardial infarction

作　　者：裴之俊[1] 李伏燕[1] 陈义加[1] 王俊杰[1] 李薇[1] 吴瑞敏[1] 杨怡[1] 

机构地区：[1]湖北医药学院附属太和医院PET中心,湖北十堰442000

出　　处：《检验医学与临床》2015年第7期937-939,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学青年基金资助项目(81401447)

摘　　要：目的建立可用于制备心肌梗死大鼠模型的新方法。方法异氟醚气体麻醉Sprague-Dawley大鼠后,钝性分离肋间肌,结扎左冠状动脉前降支,制备心肌梗死大鼠模型。以仅穿刺不结扎左冠状动脉前降支的方法制备假手术大鼠模型。术后采用小动物心电图仪、血流动力学分析仪、2,3,5-氯化三苯基四氮唑(TTC)染色及苏木素-伊红(HE)染色鉴定心肌梗死大鼠模型。结果心肌梗死大鼠模型及假手术大鼠模型存活率均为80.0%。心肌梗死模型组大鼠术后第2、7天心电图检查可见ST段明显抬高。血流动力学检测结果显示,心肌梗死模型组大鼠颈总动脉收缩压和舒张压,左心室收缩压,以及左心室压力上升和下降最大速率均较假手术模型组均明显降低,心率和左心室舒张末压则明显增加(P<0.05)。TTC染色显示梗死心肌与正常心肌色差明显,坏死心肌呈白色。HE染色显示梗死心肌细胞肿胀、排列紊乱,出现空泡,甚至断裂,部分断裂心肌细胞间隙见炎症细胞浸润。结论建立的方法效果理想且操作简单,适用于心肌梗死大鼠模型的制备。Objective To establish a new,fast and efficient preparation method of myocardial infarction model.Methods Sprague-Dawley rats were anesthetized by using isoflurane gas,then myocardial infarction models were prepared by ligation of ramus descendens anterior arteriae coronaria sinistrae,after bluntly separation of intercostal muscle.Sham operation rat models were prepared by punctuation,not ligation,of ramus descendens anterior arteriae coronaria sinistrae.Small animal electrocardiograph,hemodynamic analyzer,2,3,5-triphenyltetrazolium chloride（TTC）staining and hematoxylin-eosin（HE）staining were used to identify myocardial infarction models.ResultsSurvival rates of myocardial infarction models and sham operation models were both 80.0%.Electrocardiograph analysis indicated that ST segment was raised apparently in myocardial infarction models on 2and 7days after operation.Hemodynamic detection indicated that diastolic blood pressure,systolic blood pressure,left ventricular systolic pressure and max ascending and descending rate of left ventricular pressure in myocardial infarction models were lower than sham operation models,but heart rate and heart ventricle end diastolic pressure were higher（P〈0.05）.TTC staining showed that the color of infracted myocardium was white,which was obviously different with normal myocardium,HE staining showed that infracted cardiac muscle cells were swelling,disorderly arranged,with cavity or even break,and inflammatory cell infiltration could be found within intercellular spaces of fractured cardiac muscle cells.Conclusion Preparation method,constructed in this research,could be efficient and easily performed to establish myocardial infarction models.

关 键 词：心肌梗死 动物模型 大鼠 冠状动脉 

分 类 号：R540.45[医药卫生—心血管疾病]