脂肪血制备悬浮红细胞的质量研究  

Qualification of suspended red blood cells producted by lipid blood

作　　者：骆展鹏[1] 樊小蓉 戴宝剑 王娟娟[1] 欧阳熊妍 

机构地区：[1]重庆市血液中心,400015 [2]重庆市合川区中心血站,401520

出　　处：《检验医学与临床》2015年第7期942-944,共3页Laboratory Medicine and Clinic

摘　　要：目的研究脂肪血制备的悬浮红细胞的质量变化。方法按常规制备滤除白细胞悬浮红细胞的方法处理轻、中、重度脂肪血及正常血,检测其过滤前后红细胞渗透脆性,观察红细胞在储存期K+、Na+浓度、pH值、游离血红蛋白浓度的变化。结果中、重度组红细胞渗透脆性在过滤后明显增高,轻度组、正常组无明显变化。储存期中、重度组红细胞K+浓度、游离血红蛋白浓度明显高于轻度组和正常组(P<0.05)。Na+浓度、pH值各组间比较差异无统计学意义(P>0.05)。结论对于轻度脂肪血可采用弃其血浆,利用红细胞,而对于中、重度脂肪血应该选择报废。Objective To explore the quality change of suspended leukocyte-reduced red blood cells（SLrRBCs）prepared from lipid blood and to provide experimental evidence to develop reasonable and effective methods to reduce the scrappage of lipid blood.Methods SLrRBCs were prepared from light,moderate and severe lipid blood by conventional method,and RBC osmotic fragility was detected before and after filtering.Then the changes of the concentrations of K＋,Na＋and free Hb and the pH values of different SLrRBCs were analyzed during storage period.Results RBC osmotic fragility of moderate and severe groups were increased,but no change was observed of light and normal groups.The concentrations of K＋and free Hb of moderate and sever groups were higher than those of light and normal groups（P〈0.05）,and there were no difference of the concentration of Na＋and the pH value during storage period（P〉0.05）.Conclusion RBC of light lipid blood could be utilized,but moderate and severe lipid blood should be scrapped.

关 键 词：三酰甘油 脂肪血 悬浮红细胞 

分 类 号：R457.1[医药卫生—治疗学]