益肾平肝方对自发性高血压大鼠血压及神经肽的影响  

Effect of “Yishen Pinggan Recipe ” on blood pressure and neuropeptide in spontaneously hypertensive rats

作　　者：罗国东[1] 葛华迅 高中香 朱喜英[1] 张振永[2] 刘龙民[1] 

机构地区：[1]上海中医药大学附属普陀医院中医科,上海200062 [2]上海中医药大学附属普陀医院眼科,上海200062

出　　处：《上海中医药杂志》2015年第4期91-93,97,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市普陀区中心医院重点专科项目(2013XK156I);上海市普陀区中心医院院级课题(2013PT034)

摘　　要：目的观察益肾平肝方对SHR大鼠的血压及血清神经肽Y(NPY)、降钙素基因相关肽(CGRP)、内皮素(ET)的影响,探讨其在治疗高血压中的降血压机制。方法将21只12周龄雄性自发性高血压大鼠(SHR)随机均分为3组,即益肾平肝方组(YPR组)、贝那普利组(Bena组)、SHR组,另以同周龄7只WKY大鼠作为正常对照组(WKY组)。各组给予相应干预措施12周后,采用尾套法测量大鼠血压,用酶联免疫吸附剂测定法(ELISA)检测大鼠血清NPY、CGRP、ET的含量。结果 SHR组大鼠较WKY组收缩压明显升高(P<0.01)。给予治疗4周、8周、12周后,益肾平肝方组大鼠血压较SHR组明显下降(P<0.01);与SHR组比较,益肾平肝方组大鼠血清NPY、ET的表达量降低,CGRP表达量增加(P<0.01),且与贝那普利组比较差异无统计学意义(P>0.05)。结论益肾平肝方可明显降低SHR大鼠收缩压,其机制可能与抑制NPY、ET的表达及增强CGRP的表达相关。Objective To observe the effects of＂Yinshen Pinggan Recipe＂（ YPR） on serum neuropeptide Y（ NPY）,calcitonin gene related peptide（ CGRP）,endothelin（ ET） in spontaneously hypertensive rats（ SHRs）,and to survey its mechanisms. Methods Twenty-one twelve-week old SHRs were randomly divided into 3 groups（ group YPR,group Bena,group SHR）,seven age matched male Wistar-Kyoto rats were selected as normal control group（ group WKY）. Twelve weeks after treatment,the standard blood pressure was tested by tail-cuff measurements. The expressions of serum NPY,CGRP,ET levels were measured by commercial ELISA kits. Results The systolic blood pressure（ SBP） was significantly increased in SHRs after 4 weeks,8 weeks,12 weeks treatment. And it could be inhibited by Yinshen Pinggan Recipe. In addition,the expression of NPY and ET were significantly increased in SHRs and inhibited by YPR,which increased the expression of CGRP. Conclusion YPR can reduce the systolic blood pressure of SHR,which was associated with the inhibition of the expression of NPY,ET and the activation of the expression of CGRP.

关 键 词：自发性高血压大鼠 高血压 血清神经肽Y 降钙素基因相关肽 内皮素 

分 类 号：R285.5[医药卫生—中药学]