丙泊酚对新生小鼠海马星形胶质细胞和小胶质细胞的影响  

Effects of propofol on hippocampal astrocytes and microglia in neonatal mice

作　　者：景胜[1] 黄静[1] 包晓航[1] 周功锐 王颖[2] 杨天德[1] 

机构地区：[1]第三军医大学新桥医院麻醉科,重庆400037 [2]第三军医大学组织胚胎学教研室,重庆400038

出　　处：《重庆医学》2015年第11期1469-1471,共3页Chongqing medicine

摘　　要：目的观察丙泊酚麻醉对新生小鼠海马星形胶质细胞(AST)和小胶质细胞的影响。方法将健康同窝日龄7d(P7)C57小鼠15只,随机分为丙泊酚高剂量组、低剂量组和10%脂肪乳对照组,每组5只,所有小鼠从P7开始接受药物处理。高、低剂量组分别腹腔注射丙泊酚60、30mg/kg;对照组腹腔注射同等体积的10%脂肪乳。药物处理24h后取材,采用免疫组化方法检测AST标记物胶质纤维酸性蛋白(GFAP)与小胶质细胞标记物离子钙接头分子(Iba1)在海马内的表达。结果丙泊酚高剂量组小鼠海马齿状回分子层中GFAP标记的AST数量较对照组明显减少(P<0.01),低剂量丙泊酚对新生鼠海马中AST数量无显著影响;高剂量和低剂量丙泊酚均显著性降低海马小胶质细胞数量(P<0.01)。结论丙泊酚抑制新生小鼠海马AST和小胶质细胞的发育,且存在剂量依赖关系。Objective To observe the effects of propofol on the hippocampal astrocytes and microglia in the nenotal mice . Methods 15 healthy mice from the same litters on postnatal 7 d were randomized into 3 groups：high dose propofol group ,low dose propofol group and 10% intralipid control group .All mice were treated with drugs on postnatal 7 d by intraperitoneal injection and were sacrificed at 24 h after drugs treatment .The high dose group was injected with propofol 60mg ＆#183; kg -1 ;the low dose group was injected with propofol 30mg · kg -1 ;the control group was injected with the equal volume of 10% intralipid .The immunohistochem‐istry assay was used to detect the expression of glial fibrillary acidic protein （GFAP） and ionized calcium binding adapter molecular 1 （Iba1） for observing the effect of propofol on the astrocytes （AST ） and microglia in the hippocampus .Results Compared with the control group ,the number of GFAP‐labeled AST in the dentate gyms （DG） molecular layer of hippocampus in P7 mice of the high dose propofol group was significantly reduced （P〈0 .01） ,while no obvious effect of the low‐dose propofol on the number of AST was observed ;high dose and low dose propofol all significantly decreased the number of Iba1‐labeled microglia .Conclusion Propofol can inhibit the growth of the hippocampal AST and microglia in a dose‐dependent manner .

关 键 词：二异丙酚 海马 神经胶质 星形细胞 小神经胶质细胞 新生小鼠 

分 类 号：R726.1[医药卫生—儿科]