基于鲁米诺-过氧化氢-CdTe的流动注射-化学发光体系及应用于多巴胺的检测  

Determination of dopamine with the enhanced chemiluminescence system of luminol-H_2O_2 in the prescence of CdTe

作　　者：王金丽[1] 肖虎勇[1] 韩韵[1] 丁然[1] 吕鉴泉[1,2] 

机构地区：[1]湖北师范学院化学化工学院,湖北黄石435002 [2]嘉应学院化学与环境学院,广东梅州514015

出　　处：《湖北师范学院学报（自然科学版）》2015年第1期79-85,共7页Journal of Hubei Normal University(Natural Science)

基　　金：湖北省自然科学基金(2010CDA061);生命分析化学国家重点实验室开放基金(SKLACLS1209)资助

摘　　要：在碱性介质中,CdTe量子点对鲁米诺-过氧化氢体系有增敏作用,而多巴胺对该体系有着抑制作用。结合流动注射化学发光技术,建立了快速测定多巴胺的流动注射化学发光分析法,并对可能的机理进行了探讨。结果表明,在优化条件下,多巴胺在1.2×10^-5-7.8×10^-8mol/L浓度范围与化学发光强度呈良好的线性关系,检测限为1.5×10^-9mol/L,对于浓度为5×10^-6mol/L的多巴胺,平行测定11次的相对标准偏差为2.0%.此法成功用于尿液和制剂中多巴胺的测定。In alkaline media, CdTe quantum dots can gzeafly enhance the chemiluminescence between ]umlnol and hydrogen peroxide, however, dopamine hydrechloride can inhibit the CL of luminol- H2O2 -CdTe QDs system. Based on this characteristic, a simple and convenient method for the determination of dopamine hydrochloride was developed by combination chemiluminescence and flow - injection analysis technique. The possible CL mechanism was also discussed. Under the optimum conditions, the CL intensity was linear with the concentration of dopamine from 1.2 ×10^-5to 7.8 ×10^-8 mol/L with a detection limit（ S/N = 3） of 1.5×10^-9 mol/L. The RSD（ n = 11 ） for the detection of 2.0% method was sucessfully applied to analyze dopamine in pharmaceutical and human urine.

关 键 词：流动注射 化学发光 多巴胺 尿液 制剂 

分 类 号：O657.3[理学—分析化学]