小单孢菌40027菌株内源质粒pJTU101的特性及其抗性标记  

The Characteristic and Resistance Marker of Plasmid pJTU101 from Micoromonospora sp.40027

作　　者：李晓华[1] 杨振飞[1] 李阳[1] 陶新园 吴金龙[1] 刘涛[1] 陈鑫[1] 梅枫 马婷婷[1] 

机构地区：[1]中南民族大学生命科学学院,生物技术国家民委重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2015年第1期33-35,42,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31070087;30570046);湖北省自然科学基金重点项目(2011CDA079;2008CDB076);国家大学生创新创业训练项目(GCX12012)

摘　　要：从稀有放线菌小单孢菌40027菌株中分离到2个内源质粒p JTU101和p JTU112,用Bam H I单酶切质粒p JTU101确定其大小,通过同源片段之间发生单交换实现内源质粒p JTU101的抗性标记,并对其稳定性进行了初步研究.结果表明：小单孢菌40027菌株内源质粒p JTU101拷贝数为1-2,大小约为36.6 kb,被阿泊拉霉素抗性基因标记且在小单孢菌40027菌株中比较稳定.Two plasmids,p JTU101 and p JTU112,were isolated from Micoromonospora sp. 40027. The size of plasmid p JTU101 was identified by Bam H I restriction patterns and plasmid p JTU101 was labeled via single crossover between homologous fragments. The stability of p JTU101 was also studied. The results suggested that the plasmid p JTU101 with copy number of 1 - 2,which was about 36. 6 kb,had high genetic stability with apramycin-resistant gene marker in Micoromonospora sp. 40027.

关 键 词：小单孢菌40027菌株 质粒pJTU101 抗性基因标记 稳定性 

分 类 号：Q933[生物学—微生物学]