实时定量PCR鉴定转基因作物纯合体  

Identification of the Homozygosis of Genetically Modified Crop by Real-time Quantitative PCR

作　　者：张丽[1] 刘丽丽[1] 梁晓声[1] 王海英[1] 

机构地区：[1]中南民族大学生命科学学院武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2015年第1期43-46,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31401607;31300829);中南民族大学中央高校基本科研业务费专项资助项目(CZQ13009)

摘　　要：以水稻内标准基因磷脂酶D基因(phospholipase D,PLD)和转基因水稻TT51-1特异性旁侧序列为检测靶标,对单株转基因水稻的种子播种后长出的单株进行了荧光定量PCR,以其内标准基因和旁侧序列Ct值的差值判断了植株的纯合体,并用标准曲线计算了转基因含量,证实了此法的可靠性,说明此法用于鉴定植株的纯合体简便准确.Rice endogenous reference gene phospholipase D gene（ PLD） and genetically modified（ GM） rice TT51-1event-specific flanking sequence were used as PCR detection targets. And the homozygosis of GM rice TT51-1 plants originated from single plant seeds were analyzed through real-time PCR assays,which analyzed the △Ct value of the endogenous reference gene and the flanking sequences. The reliability of this method was verified by calculation of GM copy number ratio based on the standard curves. It was concluded that the method was simple and accurate to identify the homozygosis of GM crops.

关 键 词：转基因作物 纯合体 内标准基因 旁侧序列 实时荧光定量PCR 

分 类 号：Q788[生物学—分子生物学]