乙肝5项ELISA水浴箱与快速孵育器使用结果的可比性  

Comparability of 5 items of hepatitis B in ELISA by using water bath box and rapid incubation

作　　者：董旭才[1] 史欢欢[1] 程芳[1] 王蔓[1] 徐静[1] 张惠中[1] 

机构地区：[1]陕西省西安市唐都医院检验科,陕西西安710038

出　　处：《国际检验医学杂志》2015年第7期923-924,共2页International Journal of Laboratory Medicine

摘　　要：目的探讨及评价乙肝5项ELISA使用水浴箱与快速孵育器实验的结果比对的意义。方法随机选取本院2012年6月至2013年6月收治的乙肝患者92例,随机选取健康体检者184例作为对照,采用水浴箱和快速孵育器用ELISA同时检测标本的乙肝5项。结果 2组结果阳性符合率较高。水浴箱的标本OD值的平均值会比较高。但是快速孵育器的标本S/CO值平均值较高,与其CO值比水浴箱的标本高有关系。结论日常工作中可引进快速孵育器提高工作效率,但是"弱反应性"结果的标本,需要使用水浴箱复查,并做好记录。快速孵育器最好只用于HBsAg的反应,因其他四项的反应流程时间比较短,并且有部分结果不符合。Objective To investigate and evaluate the comparison results of five items of hepatitis B in ELISA by using water bath box and rapid incubation.Methods 92 patients with hepatitis B in our hospital from June 2012 to June 2013 were randomly selected and 184 cases of healthy physical examination were randomly selected as the controls.The water bath and rapid incubation were adopted to conduct ELISA for simultaneously detecting 5items of hepatitis B in samples.Results The results of two groups had higher positive coincidence rate.The average OD value of specimens in the water bath was higher.But the specimen S/CO value in the fast average incubation was higher,its co value was higher than that of the water bath.Conclusion The rapid incubation can be introduced for improving the work efficiency in the daily work,but the specimen with ＂ weak reactive＂ result needs to be re-detected by using the water bath and making records.The fast incubation device is best for only use of the HBsAg reaction,because the other four reaction process time is shorter,and there are some non-conformity results.

关 键 词：乙肝 水浴箱 快速孵育器 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]