实时荧光定量PCR检测75例疑似甲型流感结果分析  

Analysis of 75 cases of suspected type A influenza results by real-time fluorescent quantitative PCR test

作　　者：吴敏[1] 张立[1] 王冰冰[1] 

机构地区：[1]天津市海河医院检验科,天津300350

出　　处：《国际检验医学杂志》2015年第7期932-933,共2页International Journal of Laboratory Medicine

摘　　要：目的分析75例疑似甲型流感检测结果,了解本市流感患者分布情况,为疾病早期防控提供指导。方法采用实时荧光定量PCR的方法,检测75疑似甲型流感患者的鼻、咽拭子标本中病毒感染情况。结果 75例疑似患者标本中,共检出甲型流感病毒13例,其中H1N1亚型9例,平均年龄大于50岁。时间分布为2013年3月份3例、4月份1例,2014年1月份7例、2月份2例;未检测出H7N9亚型。结论实时荧光定量PCR具有快速、准确率高的特点,冬季为流感高发季节,老年患者为流感病毒易感人群,应及时加强监测,早期治疗。Objective To analyze the detection results of 75 suspected type A influenza cases and to understand the distribution situation of influenza patients to provide the guidance for its early prevention and control.Methods The nasopharyngeal swabs specimens from 75 patients with suspected type A influenza were detected for understanding the viral infection situation by real-time fluorescent quantitative PCR.Results In the specimens of 75 suspected patients,13 cases with type A influenza virus were detected out,in which 9cases were the H1N1 subtype,mean age 〉50 years old.The temporal distribution was 3cases in March 2013,1case in April,7cases in January 2014 and 2cases in February.No H7N9 subtype was detected out.Conclusion Real-time fluorescent quantitative PCR has the characteristics of rapidness and high accuracy.The winter is the high onset season of flu,elderly patients are susceptible to influenza virus.The monitoring and early treatment should be strengthened.

关 键 词：甲型流感 实时荧光PCR H1N1 H7N9 

分 类 号：R511.7[医药卫生—内科学] R440[医药卫生—临床医学]