硫酸镁抑制经辐射后人脐静脉血管内皮细胞γ-H2AX的表达  

MgSO4 inhibited X-rays irradiation induced expression of γ-H2AX in HUVEC

作　　者：徐华林[1] 孙阳[1] 崔凤梅[1] 涂彧[1] 

机构地区：[1]苏州大学放射医学与防护学院,215123

出　　处：《中华放射医学与防护杂志》2015年第3期161-164,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金（81372921）

摘　　要：目的 探讨不同浓度硫酸镁对照射后人脐静脉血管内皮细胞（human umbilical vein endothelial,HUVEC）存活率及γ-H2AX表达的影响.方法 CCK-8法检测不同浓度的硫酸镁对HUVEC存活率的影响;激光共聚焦显微镜检测4GyX射线照射后不同时间HUVEC中γ-H2AX簇集点（foci）的数量;流式细胞术和Western blot检测γ-H2AX蛋白的表达情况.结果 CCK-8法显示,1.25 mg/ml浓度的硫酸镁能提高照射后的细胞存活率（t=-6.34,P＜0.05）;细胞免疫荧光结果显示,照射后0.5～1 hfoci焦点数达到最高值,平均达45个,随着时间的延长loci点数变少,强度减弱,具有时间依赖性,而硫酸镁在照后0.5、1、2、6、12 h均可以明显减少loci的形成（t=12.62、6.36、11.93、5.75、9.43,P＜0.05）;流式细胞仪检测表明,硫酸镁在照后0.5、1、2h可以抑制X射线照射后γ-H2AX蛋白表达量的增加（t=6.07、5.32、11.85,P＜0.05）;Western blot结果与细胞免疫荧光结果一致.结论 硫酸镁可以增加照射后HUVEC的存活率,降低X射线诱导的DNA损伤蛋白γ-H2AX的表达.Objective To investigate the influence of MgSO4 with different concentrations on cell survival and γ-H2AX expression in HUVEC irradiated with X-rays.Methods Cell proliferation rate was assayed by CCK-8,γ-H2AX foci formationwas observed with a laser confocal microscope,and γ-H2AX protein expression was detected by flow cytometer and Western blot assay.Results MgSO4 with a concentration of 1.25 mg/ml could improve the survival rate of IHUVEC treated with X-rays （t =-6.34,P 〈 0.05）.After irradiation,γ-H2AX loci approached to the highest level from 30 min to 1 h after radiation and then decreased.MgSO4 significantly reduced the foci formation at 0.5,1,2,6 and 12 h postirradiation （t =12.62,6.36,11.93,5.75,9.43,P 〈 0.05）.Both flow cytometry,and Western blot assays showed that MgSO4 inhibited γ-H2AX protein expression at 0.5,1 and 2 h post-irradiation （t =6.07,5.32,11.85,P 〈 0.05）.Conclusions MgSO4 could improve the survival rate and reduced γ-H2AX expression in HUVEC irradiated with X-ray.

关 键 词：硫酸镁 人脐静脉血管内皮细胞 X射线 CCK-8 Γ-H2AX 

分 类 号：R811[医药卫生—放射医学]