X射线照射对小鼠辅助性T细胞相关细胞因子的影响  

Effects of X-irradiation on the T-helper cell related cytokines in mouse

作　　者：周睿卿[1,2] 何慧清[1] 郭子文[1] 牛晓敏[1] 李卫华[1] 邱大发[1] 许晓军[1] 王静[3] 

机构地区：[1]中山大学附属中山医院血液内科,广州528403 [2]广州市第一人民医院 [3]南方医科大学附属珠江医院血液内科

出　　处：《中华放射医学与防护杂志》2015年第3期183-186,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：广东省科技计划项目[粤科社字（2011）106号];中山市科技计划重大专项（20113A002）;中山市科技计划（20122A111）资助项目

摘　　要：目的 观察不同剂量X射线照射对小鼠辅助性T细胞相关细胞因子的影响.方法 100只BALB /c小鼠用随机数字表法分为4组,包括健康对照组和X射线照射组（分别予2、4和6 Gy照射）,每组25只.于照射前、照射后7、14、21和28 d,对小鼠一般情况进行观察,对外周血进行白细胞计数,用蛋白芯片检测血清中辅助性T细胞相关细胞因子含量,并用ELISA确证细胞因子的浓度变化.结果 照射后,小鼠外周血白细胞14d降至最低,同一时间点的下降程度随照射剂量增加而增加（F=86.267,P＜0.05）.芯片法初筛提示,细胞因子IFN-IFTGF-β、IL-6、IL-17上调,IL-5、IL-23、TNF-α下调.ELISA检测可见,IL-17浓度随辐射剂量增高而明显增高,6 Gy组在7、14和21 d均明显高于对照（t=23.743、21.759和17.662,P＜0.05）;IFN-γ/IL-4浓度在2和4 Gy照射后无明显变化,6 Gy照射后在7、14、21和28 d均比健康对照组明显增高（t=8.335、9.982、6.990和3.074,P＜0.05）,呈先升高后降低的变化趋势,14 d达高峰.结论 低于致死剂量的X射线照射可使小鼠血清IFN-γ 、TGF-β、IL-6、IL-17细胞因子浓度升高,呈Thl细胞偏移.Objective To study the effects of X-ray irradiation at different doses on the expressions of the T-helper cell related cytokines in mouse.Methods 100 BALB/c mice were randomly divided into four groups （25 mice in each） with 0,2,4 and 6 Gy X-ray irradiation.At 1,7,14,21 and 28 d after irradiation,the general situation of mice were observed,and the peripheral white blood cells were counted.The alterations of the serum helper T cell related cytokines were screened by protein chips and tested by ELISA.Results At 14 d after irradiation,the peripheral white blood cells of mice decreased to the lowest level.The number of peripheral white blood cells decrease along with the irradiation dose （Fdose =86.267,P 〈0.05）.There were significant increases in the release of IFN-γ,TGF-β,L-6 and IL-17,but decreases in cytokine IL-5,IL-23 and TNF-α.ELISA analysis revealed that the increases of IL-17 correlated with the irradiation dose positively and the concentration of IL-17 in 6 Gy group were higher than control group at 1,7,14 and 21 d after irradiation （t =23.743,21.759 and 17.662,P 〈 0.05）.The ratio of IFN-γ/IL-4 significantly higher than the control group at 1,7,14,21 and 28 d after irradiation （t =8.335,9.982,6.990 and 3.074,P 〈 0.05）,and it approached to the highest level at 14 d and then began to decrease.Conclusions A low dose X-irradiation induces T-helper cell related cytokines IFN-γ and IL-17,and leads to Th1-biased immune responses in mice in vivo.

关 键 词：X射线 辅助性T细胞 细胞因子 干扰素-Γ 白介素-17 

分 类 号：R818[医药卫生—放射医学]