瞬时感受器电位通道1在臭氧致小鼠肺组织炎症中的表达  

Expression of transient receptor potential canonical 1 in ozone- induced inflammatory lung tissues in mice

作　　者：扶招弟 周丽芬[1] 黄建荣[2] 郭淑仪 章洁纯 方永标 刘筱蔼[1] 陈庆梓 李建华[1] 

机构地区：[1]广州医科大学生理学教研室,广东广州510182 [2]中山大学附属第二医院骨科,广东广州510120 [3]广州医科大学第三临床学院,广东广州510182

出　　处：《南方医科大学学报》2015年第2期284-287,291,共5页Journal of Southern Medical University

基　　金：广州市属高校科研计划项目(2012C043);广东省医学科研基金(A2013246)

摘　　要：目的探讨经典瞬时感受器电位通道1(TRPC1)在臭氧暴露下小鼠肺组织中的表达情况。方法采用臭氧暴露建立小鼠肺组织炎症模型,通过对小鼠行为学观察、支气管肺泡灌洗液的细胞分类计数和肺组织病理形态分析证实模型建立成功后,RTPCR法检测肺组织TRPC1 m RNA的表达,Western blot法和免疫组织化学染色法分别观察TRPC1蛋白在肺组织的表达和定位情况。结果 RT-PCR和Western blot结果显示,与对照组相比,臭氧组小鼠肺组织TRPC1 m RNA及其蛋白表达上调(P<0.05);免疫组织化学染色法进一步证实,臭氧组小鼠肺组织TRPC1蛋白在肺泡上皮细胞、支气管上皮细胞、浸润的炎症细胞的表达均较对照组明显增强(P<0.05);相关性分析显示,小鼠肺组织中TRPC1 m RNA和蛋白的表达均与支气管肺泡灌洗液中白细胞总数、巨噬细胞、中性粒细胞、淋巴细胞数呈显著性正相关(P<0.01)。结论 TRPC1可能与小鼠肺组织炎症发病机制有关。Objective To detect the expression of transient receptor potential canonical 1（TRPC1） in a mouse model of ozoneinduced lung inflammation and explore its role in lung inflammation. Methods In a mouse model of lung inflammation established by ozone exposure, the expression of TRPC1 in the inflammatory lung tissues was detected by RT- PCR, Wstern blotting and immunohistochemistry. Results Compared to the control mice, the mice exposed to ozone showed significantly increased expression level of TRPC1 m RNA and protein in the inflammatory lung tissues（P〈0.05）. Immunohistochemistry showed increased TRPC1 protein expressions in the alveolar epithelial cells, bronchial epithelial cells, and inflammatory cells in the inflammatory lung tissues（P〈0.05）. The m RNA and protein expression levels of TRPC1 were positively correlated with the counts of white blood cells, macrophages, neutrophils and lymphocytes in the bronchoalveolar lavage fluid of the exposed mice（P〈0.01）. Conclusion TRPC1 may play a role in ozone-induced lung inflammation in mice.

关 键 词：瞬时感受器电位通道1 臭氧 肺 炎症 

分 类 号：R563.1[医药卫生—呼吸系统]