不同临床时期乙型肝炎病毒感染者血清病毒核糖核酸水平的比较  

Analysis of serum HBV RNA in different clinical stages of patients with hepatitis B

作　　者：贾雯 计焱焱 张继明 

机构地区：[1]上海静安区中心医院感染科,200040 [2]上海静安区中心医院免疫科,200040 [3]华山医院感染科

出　　处：《肝脏》2015年第1期4-7,共4页Chinese Hepatology

基　　金：上海市卫生局青年科研项目(20114Y088);上海市卫生和计划生育委员会第一批青年医师培养资助计划

摘　　要：目的观察和分析慢性HBV感染者不同临床时期的血清乙型肝炎病毒核糖核酸(HBV RNA)、HBV DNA以及HBsAg水平。方法入组86例慢性HBV感染者,抽提血清HBV RNA并以Oligo(dT)为引物反转录mRNA为cDNA,Taqmen荧光定量PCR法检测cDNA。将慢性HBV感染者不同临床时期的测定结果进行统计分析。结果血清HBV RNA定量在免疫耐受期、免疫清除期、再活动期、非活动或低(非)复制期患者中依次降低,不同临床时期血清HBV RNA水平两两比较均差异有统计学意义(P<0.05)。在慢性HBV感染者中,血清HBV RNA与HBV DNA呈正相关(HBeAg阳性,r=0.523,P=0.004;HBeAg阴性,r=0.340,P=0.034)。血清HBV RNA水平与HBsAg水平均呈正相关(HBeAg阳性,r=0.548,P<0.01;HBeAg阴性,r=0.317,P=0.049)。结论随着慢性HBV感染病程的进展,血清HBV RNA水平逐渐下降。且血清HBV RNA水平与HBV DNA、HBsAg水平呈正相关。Objective To investigate the correlation of serum hepatitis B virus（HBV）RNA,HBV DNA and HBsAg levels in patients with hepatitis B. Methods Eighty-six patients with persistent HBV-infection were enrolled.For measurement of serum HBV RNA,total nucleic acid was extracted from serum samples and treated with RNase-free DNase I.After cDNA synthesis from extracted RNA,HBV RNA was measured by real-time PCR.The first strand of cDNA was synthesized with an oligo （dT）primer.Results Serum HBV RNA levels reduced sequentially from immune tolerance phase,immune clearance phase,reactivity phase,to inactivity phase,and the difference between any two phases was statistically significant （P 〈 0.05）.Furthermore,we found positive correlation between serum HBV RNA and HBV DNA in patients with persistent HBV-infection （HBeAg positive,r = 0.523,P = 0.004,HBeAg negative,r = 0.340,P = 0. 034）.There were also positive correlations between serum HBV RNA and HBsAg （HBeAg positive,r=0.548,P 〈0.001 , HBeAg negative,r= 0.317,P = 0.049）.Conclusion As the progression of persistent HBV-infection,seurm HBV RNA levels presented the gradually decreasing tendency.Our results confirmed a strong positive correlation between serum HBV RNA and HBsAg,serum HBV RNA and HBV DNA levels in patients with persistent HBV-infection.

关 键 词：乙型肝炎病毒感染 HBV RNA定量 HBSAG定量 HBV DNA定量 

分 类 号：R512.62[医药卫生—内科学]