阿托伐他汀对自发性高血压大鼠心肌组织p21表达的影响及其意义  

Effects of atorvastatin on p21 expression in hypertrophic myocardium of spontaneously hypertensive rats

作　　者：赵丽丽[1] 蒲丽君[1] 赵珂[1] 罗勇[1] 

机构地区：[1]川北医学院附属医院心内科,四川南充637000

出　　处：《心脏杂志》2015年第2期134-138,141,共6页Chinese Heart Journal

摘　　要：目的:观察阿托伐他汀(ATV)对自发性高血压大鼠(SHR)心肌组织中p21表达的影响,探讨其改善心肌肥厚的可能机制。方法:将16只8周龄SHR随机分为2组(n=8):ATV药物干预组(ATV组)与SHR模型对照组(SHR组),并以8只同周龄Wistar-Kyoto大鼠作为正常对照组(WKY组)。ATV组用ATV 50 mg/(kg·d)灌胃,SHR组与WKY组采用等容量蒸馏水每日同时灌胃。每隔2周测1次血压。10周后,观察大鼠血脂、心肌肥厚指标、p21 mRNA及其蛋白表达的改变。结果:干预10周后,ATV组及SHR组血脂、血压无明显差异。ATV组左室质量指数低于SHR组(P<0.01)。ATV组p21mRNA及蛋白的表达明显高于SHR组(P<0.01)。心肌组织p21mRNA的表达与全心质量与体质量比(HW/BW)呈负相关(r=-0.709,P<0.01),与左室质量与体质量比(LVW/BW)呈负相关(r=-0.665,P<0.01)。结论:ATV可上调SHR肥厚心肌组织中p21的表达,可有效改善左室肥厚。AIM： To examine the effect of atorvastatin on p21 expression of left ventricular hypertrophy in spontaneously hypertensive rats（ SHR） and the underlying mechanisms of atorvastatin in alleviating myocardial hypertrophy. METHODS： Sixteen 8-week-old male SHR were randomly divided into ATV group（ n = 8） and SHR group（ n = 8）. Age-matched Wistar-Kyoto rats served as normotensive control group（ WKY group,n = 8）. In ATV group,atorvastatin was given at a dose of 50 mg / kg per day by gastric gavage. In the SHR group and WKY group,the same volume of distilled water was given. Blood pressure was measured every 2 weeks and after 10 weeks,plasma lipid levels were measured. Ventricular weight index was calculated by the ratio of left ventricular weight to body weight and p21 expressions were examined by immunohistochemistry analysis and RT-PCR. RESULTS： No significant difference was found in plasma lipid levels and blood pressure between ATV group and SHR group. Compared with that in the SHR group,left ventricular weight index decreased significantly in the ATV group（ P〈0. 01） and the myocardium p21 expression increased significantly（ P〈0. 01）. The expression of p21 mRNA in cardiomyocytes were negatively correlated with HW / BW（ r =-0. 709,P〈0. 01） and LVW / BW（ r =-0. 665,P〈0. 01）. CONCLUSION： Atorvastatin increases myocardium P21 expression and alleviates left ventricular hypertrophy in spontaneously hypertensive rats.

关 键 词：阿托伐他汀 自发性高血压大鼠 心肌肥厚 P21 大鼠 

分 类 号：R541.3[医药卫生—心血管疾病]