白藜芦醇对大鼠海马缺血再灌注损伤的保护机制  

Effect of resveratrol on hippocampal glutamate of brain in ischemia/reperfusion rats

作　　者：赵刚[1,2] 王伟民[2] 杨欢[2] 刘晓燕[2] 杨帅[2] 钟世镇[1] 

机构地区：[1]南方医科大学解剖学教研室,广东广州510515 [2]广州军区广州总医院神经外科,广东广州510010

出　　处：《哈尔滨医科大学学报》2015年第1期27-29,44,共4页Journal of Harbin Medical University

基　　金：广东省自然科学基金资助项目(7000055)

摘　　要：目的研究白藜芦醇（resveratrol,Res）对脑缺血再灌注（ischemia/reperfusion,I/R）损伤保护作用的可能机制。方法 90只大鼠随机平均分为9组,假手术组（A组）、术后持续缺血组（B1组）和缺血再灌注后丙二醇溶液组（B2组）、持续缺血后Res干预组（C1~C3组）和缺血再灌注后Res干预组（D1~D3组）,Res浓度分别为10~8 g/kg、10~7 g/kg、10~6 g/kg。大鼠脑缺血2 h后再灌注,之后Res治疗3天。反相高效液相层析检测海马组织谷氨酸（glutamate,Glu）含量,免疫组化方法测定谷氨酸/天冬氨酸转运体（glutamate/aspartate transporter,GLAST）及谷氨酸转运体-1（glutamate transporter-1,GLT-1）蛋白阳性的表达。结果与B1~B2组比较,C1~C3组和D1~D3组Glu含量明显降低,同时GLAST和GLT-1的表达升高。结论 Res通过提高GLAST和GLT-1的表达,降低Glu含量,起到脑缺血再灌注损伤后的神经保护作用。Objective To explore the effect of resveratrol（ Res） on hippocampal of brain in ischemia / reperfusion rats. Methods The middle cerebral artery occlusion models of rats were made. Ninety male Sprague-Dawley rats were randomly divided into 9 groups（ n = 10） ： sham operation group（ A group）,continuing ischemia group（ B1 group） and propylene glycol solution after reperfusion group（ B2 group）,Res after continuing ischemia group（ C1 ~ C3groups） and Res after reperfusion group（ D1 ~ D3 groups）,and the dose of Res was 10 ~ 8 g /kg,10 ~ 7 g / kg,10 ~ 6 g / kg respectively. 3d Res treatment after reperfusion,reverse phase high-performance liquid chromatography（ RP-HPLC） was used to examine the glutamate concentration of the ischemic brain tissue homogenate,and immunohistochemistry was used to examine the expression of glutamate / aspartate transporter（ GLAST） and glutamate transporter-1（ GLT-1）. Results Compared with B1 ~ B2 groups,the glutamate concentration was significantly decreased（ P 0. 05）,and the expression of GLAST and GLT-1 was increased in C1 ~C3 groups and D1 ~ D3 groups. Conclusion The mechanism of neuroprotective effects of Res on focal cerebral ischemia / reperfusion injury may by increasing the expression of GLAST and GLT-1 in the brain tissue and inhibiting the expression of glutamate.

关 键 词：白藜芦醇 缺血再灌注 谷氨酸 谷氨酸/天冬氨酸转运体 谷氨酸转运体-1 

分 类 号：R282.710.5[医药卫生—中药学]